| 產(chǎn)品編號 | bs-3738R-HRP |
| 英文名稱 | Rabbit Anti-phospho-Crkl (Tyr251)/HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標記的磷酸化接頭蛋白CrkL抗體 |
| 別 名 | Crk L; Crk like protein; Crkol; HGNC:2363; Oncogene CrkL; V crk avian sarcoma virus CT10 oncogene homolog like; V crk sarcoma virus CT10 oncogene homolog avian like; CRKL_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 34kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Crkl around the phosphorylation site of Tyr251 [CA(p-Y)D] |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Crkl is a 34kDa adaptor protein which has been shown to activate the RAS and JUN kinase signalling pathways and transforms fibroblasts in a RAS dependant manner. Crkl is a substitute of BCR ABL tyrosine kinase. In addition, Crkl has oncogenic potential. Function: May mediate the transduction of intracellular signals. Subunit: Interacts with INPP5D/SHIP1. Interacts with DOCK2 and EPOR. Interacts with phosphorylated CBLB and IRS4. Similarity: Belongs to the CRK family. Contains 1 SH2 domain. Contains 2 SH3 domains. Database links: Entrez Gene: 1399 Human Entrez Gene: 12929 Mouse Omim: 602007 Human SwissProt: P46109 Human SwissProt: P47941 Mouse Unigene: 5613 Human Unigene: 21048 Mouse Unigene: 451241 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 接頭蛋白CrkL是Crk家族成員之一，ＣＲＫＬ為具有ＳＨ２及ＳＨ３結(jié)構(gòu)的連接蛋白，CrkL參與多種信號轉(zhuǎn)導(dǎo)途徑調(diào)節(jié)細胞運動、增殖及分化,在各種組織中廣泛表達,在多種信號途徑中起重要作用。 |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
