| 產品編號 | bs-2574R-HRP |
| 英文名稱1 | Rabbit Anti-GCSF Receptor/HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標記的粒細胞-巨噬細胞集落刺激因子3受體抗體 |
| 別 名 | GCSF Receptor; CD 114; CD114; CD114 antigen; Colony stimulating factor 3 receptor (granulocyte); Colony stimulating factor 3 receptor; CSF 3R; CSF3R; CSF3R_HUMAN; Csfgr; G CSF R; G-CSF receptor; G-CSF-R; G-CSFR; GCSFR; Granulocyte colony stimulating factor receptor; Granulocyte colony stimulating factor receptor precursor; Granulocyte colony-stimulating factor receptor. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 免疫學 發(fā)育生物學 信號轉導 干細胞 生長因子和激素 細胞膜受體 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, (predicted: Mouse, Rat, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 95kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human G-CSFR |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: The protein encoded by the GCSF Receptor gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variants encoding four different isoforms have been found for this gene, of which three are membrane-bound and the one secreted and soluble. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. Function: Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface. Subunit: Homodimer. The dimeric receptor binds two CSF3 molecules. Subcellular Location: Isoform 2: Secreted (Probable). Cell membrane; Single-pass type I membrane protein. Tissue Specificity: One or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U937 cells. Isoform GSCFR-3 is highly expressed in placenta. DISEASE: Defects in CSF3R are the cause of hereditary neutrophilia (NEUTROPHILIA) [MIM:162830]. A form of lifelong, persistent neutrophilia, a condition characterized by an increase in the number of neutrophils in the blood. Similarity: Belongs to the type I cytokine receptor family. Type 2 subfamily. Contains 5 fibronectin type-III domains. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Database links: Entrez Gene: 1441 Human Entrez Gene: 12986 Mouse Omim: 138971 Human SwissProt: Q99062 Human SwissProt: P40223 Mouse Unigene: 524517 Human Unigene: 271701 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
