產品編號 | bs-1537R-Gold |
英文名稱1 | Rabbit Anti-NADPH/Gold Conjugated antibody |
中文名稱 | 膠體金標記的還原型輔酶Ⅱ/磷酸酰胺腺嘌呤二核苷酸抗體 |
別 名 | nicotinamide adenine dinucleotide phosphate; NADP; NADPH; TPNH; Triphosphopyridine nucleotide, reduced form; |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul(10nm 15nm 35nm) |
產品類型 | 小分子抗體 |
研究領域 | 腫瘤 細胞生物 免疫學 信號轉導 激酶和磷酸酶 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | |
產品應用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 0.833kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 0.4mg/ml |
免 疫 原 | KLH conjugated NADPH |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
保存條件 | Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles. |
產品介紹 |
background: NDAPH (nicotinamide adenine dinucleotide phosphate) includes of Alpha-NADPH and Beta-NADPH. Beta-NADPH hase activity only. Beta-Nicotinamide adenine dinucleotide 2'-phosphate reduced tetrasodium salt or triphosphopyridine nucleotide reduced tetrasodium salt, also known as Coenzyme Ⅱ. β-Nicotinamide adenine dinucleotide 2′-phosphate (NADP+) and β-Nicotinamide adenine dinucleotide 2′-phosphate, reduced (NADPH) comprise a coenzyme redox pair (NADP+:NADPH) involved in a wide range of enzyme catalyzed oxidation reduction reactions. The NADP+/NADPH redox pair facilitates electron transfer in anabolic reactions such as lipid and cholesterol biosynthesis and fatty acyl chain elongation. The NADP+/NADPH redox pair is used in a variety of antioxidation mechanism where it protects agains reactive oxidation species accumulation. NADPH is generated in vivio by the pentose phosphate pathway (PPP). Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. NADPH氧化酶廣泛存在于機體多個系統(tǒng)發(fā)揮重要的生理功能。在多種病理情況下,NADPH氧化酶可以在很多組織中催化合成大量活性氧,導致過度氧化應激,使組織細胞的功能下降。 NADPH氧化酶也是血管內皮細胞ROS產生的主要酶系,由其介導產生ROS在信息傳遞、基因轉錄、細胞生長和凋亡以及調節(jié)內皮依賴性的一氧化氮合酶脫偶聯等血管舒張中的作用,使其成為血管生物學中重要的部分,NADPH氧化酶一方面與血管舒張的機能異常有關,另一方面又影響到內皮細胞的結構、粘附分子的表達、滲透、成長及遷移等功能。 |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |