| 產(chǎn)品編號 | bs-55145R |
| 英文名稱 | NDUFB4 Rabbit pAb |
| 中文名稱 | NDUFB4蛋白抗體 |
| 別 名 | B15; CI-B15; Complex I B15 subunit; Complex I-B15; NADH dehydrogenase(ubiquinone) 1 beta subcomplex 4 15kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4; NADH ubiquinone oxidoreductase B15 subunit; NADH-ubiquinone oxidoreductase B15 subuni |
| 研究領(lǐng)域 | 腫瘤 細胞生物 信號轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 15 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human NDUFB4 protein: 1-90/129 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | PBS with 0.02% Proclin300,50% Glycerol,pH7.3. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone. [provided by RefSeq, Dec 2009] SWISS: O95168 Gene ID: 4710 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Mouse Kidney tissue lysates
Lane 3: Human HeLa cell lysates
Lane 4: Human MOLT4 cell lysates
Lane 5: Human U87MG cell lysates
Lane 6: Human 293T cell lysates
Lane 7: Human U-2 OS cell lysates
Primary: Anti-NDUFB4 (bs-55145R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
|
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
