| 產(chǎn)品編號 | bsm-51656M |
| 英文名稱 | Mouse Anti-EIF2B3 antibody |
| 中文名稱 | eIF2Bγ單克隆抗體 |
| 別 名 | EI2BG_HUMAN; EIF 2B; EI2BG; eIF 2B GDP GTP exchange factor subunit gamma; eIF-2B GDP-GTP exchange factor subunit gamma; eIF2B-gamma; EIF2Bgamma; Eukaryotic translation initiation factor 2B subunit 3 gamma; Eukaryotic translation initiation factor 2B, gamma; eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa; OTTHUMP00000010262; OTTHUMP00000010263; Translation initiation factor eIF-2B subunit gamma. |
| 研究領(lǐng)域 | 細胞生物 神經(jīng)生物學 表觀遺傳學 Alzheimer's |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | H11F4 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:25,ICC/IF=1:25,IF=1:25
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EIF2B3: 151-400/452 |
| 亞 型 | IgG1,k |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] Function: Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Subunit: Complex of five different subunits; alpha, beta, gamma, delta and epsilon. DISEASE: Defects in EIF2B3 are a cause of leukodystrophy with vanishing white matter (VWM) [MIM:603896]. VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy. Similarity: Belongs to the eIF-2B gamma/epsilon subunits family. SWISS: Q9NR50 Gene ID: 8891 Database links: Entrez Gene: 8891 Human SwissProt: Q9NR50 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: HL-60 cell lysates
Lane 2: MCF-7 cell lysates
Lane 3: U-2OS cell lysates
Lane 4: K562 cell lysates
Primary: Anti-EIF2B3 (bsm-51656M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 56 kD
U-2 OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (EI2BG) monoclonal Antibody, Unconjugated (bsm-51656M) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.Dylight? 554 Phalloidin (red) was used to stain the cell Cytoplasmic actin.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
