| 產(chǎn)品編號 | bs-19997R |
| 英文名稱 | Rabbit Anti-PACE4 antibody |
| 中文名稱 | PACE4蛋白抗體 |
| 別 名 | Paired basic amino acid cleaving enzyme 4; paired basic amino acid cleaving system 4; PCSK6; PCSK6_HUMAN; Proprotein convertase subtilisin/kexin type 6; SPC4; Subtilisin like protease; Subtilisin-like proprotein convertase 4; subtilisin/kexin like protease PACE4; Subtilisin/kexin-like protease PACE4. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 神經(jīng)生物學(xué) 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 91kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PACE4: 711-810/969 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to the trans-Golgi network where a second autocatalytic event takes place and the catalytic activity is acquired. The encoded protease is constitutively secreted into the extracellular matrix and expressed in many tissues, including neuroendocrine, liver, gut, and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include transforming growth factor beta related proteins, proalbumin, and von Willebrand factor. This gene is thought to play a role in tumor progression and left-right patterning. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2014] Function: Likely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif. Subcellular Location: Secreted; Endomembrane system. Retained intracellularly probably through a hydrophobic cluster in their C-terminus and Endoplasmic reticulum. Not secreted, remains probably in zymogen form in endoplasmic reticulum. Tissue Specificity: Each PACE4 isoform exhibits a unique restricted distribution. Isoform PACE4A-I is expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, but at comparatively higher levels in the liver. Isoform PACE4A-II is at least expressed in placenta. Isoform PACE4B was only found in the embryonic kidney cell line from which it was isolated. Isoform PACE4C and isoform PACE4D are expressed in placenta. Isoform PACE4E-I is expressed in cerebellum, placenta and pituitary. Isoform PACE4E-II is at least present in cerebellum. Post-translational modifications: The PACE4A-I precursor protein seems to exist in the reticulum endoplasmic as both a monomer and a dimer-sized complex whereas mature PACE4A-I exists only as a monomer, suggesting that propeptide cleavage affects its tertiary or quaternary structure. Similarity: Belongs to the peptidase S8 family. Contains 1 homo B/P domain. Contains 1 PLAC domain. SWISS: P29122 Gene ID: 5046 Database links: Entrez Gene: 100673009 Elephant Entrez Gene: 100057677 Horse Entrez Gene: 5046 Human Entrez Gene: 100405129 Marmoset (common) Entrez Gene: 18553 Mouse Entrez Gene: 100020955 Opossum Omim: 167405 Human SwissProt: P29122 Human Unigene: 498494 Human Unigene: 665989 Human Unigene: 950 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Rat Testis tissue lysates
Lane 2: Human 293T cell lysates
Lane 3: Human HepG2 cell lysates
Lane 4: Human A549 cell lysates
Lane 5: Human SH-SY5Y cell lysates
Primary: Anti-PACE4 (bs-19997R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 91 kDa
Observed band size: 135 kDa
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