| 產(chǎn)品編號 | bs-17894R |
| 英文名稱 | Rabbit Anti-mtRNA polymerase antibody |
| 中文名稱 | 線粒體RNA聚合酶抗體 |
| 別 名 | APOLMT; DNA-directed RNA polymerase; DNA-directed RNA polymerase mitochondrial; h-mtRPOL; mitochondrial; MTRNAP; MtRPOL; POLRMT; polymerase (RNA) mitochondrial (DNA directed); polymerase, RNA, mitochondrial; RPOM; RPOM_HUMAN. |
| 研究領域 | 細胞生物 轉錄調節(jié)因子 新陳代謝 線粒體 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 134kDa |
| 細胞定位 | 細胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human mtRNA polymerase: 951-1050/1230 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. [provided by RefSeq, Jul 2008] Function: DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates. Subunit: Interacts with TFB1M and TFB2M, leading to the stimulation of transcription. Interacts with TEFM. Subcellular Location: Mitochondrion. Similarity: Belongs to the phage and mitochondrial RNA polymerase family. SWISS: O00411 Gene ID: 5442 Database links: Entrez Gene: 100723303 Guinea pig Entrez Gene: 5442 Human Omim: 601778 Human SwissProt: O00411 Human Unigene: 254113 Human Unigene: 24623 Rat |
| 產(chǎn)品圖片 |
Sample:
Jurkat(Human) Cell Lysate at30 ug
Primary: Anti-mtRNA polymerase (bs-17894R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 134 kD
Observed band size: 134 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
