| 產(chǎn)品編號 | bs-14198R |
| 英文名稱 | Rabbit Anti-DARS2 antibody |
| 中文名稱 | 天門冬氨酰tRNA連接酶2抗體 |
| 別 名 | Aspartate tRNA ligase 2 mitochondrial; Aspartate tRNA ligase; Aspartate tRNA ligase mitochondrial; Aspartyl tRNA synthetase 2 (mitochondrial); Aspartyl tRNA synthetase mitochondrial; AspRS; FLJ10514; SYDM_HUMAN; LBSL; MT ASPRS; RP3 383J4.2. |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) 新陳代謝 線粒體 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Cow) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 68kDa |
| 細胞定位 | 細胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DARS2: 551-645/645 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). [provided by RefSeq, Nov 2009] Function: Aspartyl-tRNA synthetase (DARS) charges its cognate tRNA with aspartate during protein biosynthesis. Mammalian synthetases differ from those of prokaryotes and lower eukaryotes in that they associate as multienzyme complexes. Defects in DARS2 are a cause of leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation. Subcellular Location: Mitochondrion matrix. Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. SWISS: Q6PI48 Gene ID: 55157 Database links: Entrez Gene: 55157 Human Entrez Gene: 226539 Mouse GenBank: NM_018122.3 Human Omim: 610956 Human SwissProt: Q6PI48 Human SwissProt: Q8BIP0 Mouse |
| 產(chǎn)品圖片 |
Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-DARS2 Polyclonal Antibody, Unconjugated(bs-14198R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
