| 產(chǎn)品編號(hào) | bs-7713R |
| 英文名稱 | Rabbit Anti-MIS12 antibody |
| 中文名稱 | 染色體及有絲分裂蛋白MIS12抗體 |
| 別 名 | hMIS12; homolog of yeast Mis12; KNTC2AP; MIND kinetochore complex component, homolog (yeast); MIS12 homolog (yeast); MIS12 homolog; MIS12 MIND kinetochore complex component homolog (yeast); MTW 1; MTW1; Protein MIS12 homolog; MIS12_HUMAN. |
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Specific References (3) | bs-7713R has been referenced in 3 publications.
[IF=12.511] Ogasawara et al. Cathepsin K activity controls cardiotoxin-induced skeletal muscle repair in mice. (2018) J.Cachexia.Sarcopenia.Muscle. 9:160-175 IHC-P ; Mouse.
[IF=9.6] Ogasawara, Shinyu, et al. "Cathepsin K activity controls cardiotoxin‐induced skeletal muscle repair in mice." Journal of Cachexia, Sarcopenia and Muscle (2017). IHC-P ; Mouse.
[IF=5.443] Goto Hiroki. et al. Proliferin-1 Ameliorates Cardiotoxin-Related Skeletal Muscle Repair in Mice. Stem Cells Int. 2021;2021:9202990 IF ; Mouse.
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| 研究領(lǐng)域 | 細(xì)胞生物 細(xì)胞周期蛋白 細(xì)胞分化 表觀遺傳學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 24kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MIS12: 11-120/205 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
MIS12 is part of a complex that plays an essential role in chromosome segregation in vertebrates and contributes to mitotic kinetochore assembly. Function: Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. Subunit: Component of the MIS12 complex composed of MIS12, DSN1, NSL1 and PMF1. Also interacts with CASC5, CBX3, CBX5, NDC80 and ZWINT. Subcellular Location: Nucleus. Chromosome, centromere, kinetochore. Note=Associated with the kinetochore. Similarity: Belongs to the mis12 family. SWISS: Q9H081 Gene ID: 79003 Database links: UniProtKB/Swiss-Prot: Q9H081.1 細(xì)胞周期是細(xì)胞最基本的生命活動(dòng)之一,它通過(guò)一系列有序的反應(yīng)進(jìn)行分裂,產(chǎn)生兩個(gè)包含相同遺傳物質(zhì)的子細(xì)胞。細(xì)胞周期中最核心的事件就是進(jìn)行遺傳物質(zhì)的復(fù)制和分離,即在S期進(jìn)行DNA的復(fù)制,然后在有絲分裂時(shí)期將復(fù)制的染色體平均分配到子細(xì)胞中去。有絲分裂過(guò)程中染色體的運(yùn)動(dòng)和分離又是通過(guò)紡錘體微管和位于染色體著絲粒上的動(dòng)點(diǎn)之間的協(xié)同作用來(lái)完成的。 MIS12是一個(gè)進(jìn)化上較為保守的蛋白復(fù)合物,人源MIS12復(fù)合物由Mis13、Mis14、Mis12和PMF1四個(gè)蛋白組成,功能性研究表明它們?cè)趧?dòng)點(diǎn)的組裝和染色體的分離中發(fā)揮重要作用,但是，目前它們動(dòng)點(diǎn)定位機(jī)制仍不清楚，還有待進(jìn)一步研究。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MIS12) Polyclonal Antibody, Unconjugated (bs-7713R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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