| 產(chǎn)品編號 | bs-7616R |
| 英文名稱 | MCG10 Rabbit pAb |
| 中文名稱 | RNA相關結合蛋白MCG10抗體 |
| 別 名 | alpha CP4; LIP4; LYST interacting protein; PCBP4; poly(rC) binding protein 4; rCbinding protein 4; RNA binding protein MCG10; PCBP4_HUMAN. |
| 研究領域 | 細胞生物 信號轉導 細胞凋亡 細胞周期蛋白 轉錄調(diào)節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MCG10: 1-100/403 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
MCG10 is a member of the KH-domain RNA-binding protein family. Proteins of this family bind to RNA with specificity for C-rich pyrimidine regions. MCG10 is induced by p53 and DNA damage via two p53-responsive promoter elements. Function: Single-stranded nucleic acid binding protein that binds preferentially to oligo dC Subcellular Location: Cytoplasm. Similarity: Contains 3 KH domains. SWISS: P57723 Gene ID: 57060 Database links: Entrez Gene: 57060 Human Entrez Gene: 59092 Mouse Omim: 608503 Human SwissProt: P57723 Human SwissProt: P57724 Mouse Unigene: 20930 Human |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with MCG10 polyclonal antibody, unconjugated (bs-7616R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
