| 產(chǎn)品編號 | bs-6090R |
| 英文名稱 | LUCA15 Rabbit pAb |
| 中文名稱 | 腫瘤抑制基因LUCA15抗體 |
| 別 名 | G15 antibody H37 antibody LUCA 15; LUCA15; Protein G15; RBM 5; Renal carcinoma antigen NY REN 9; RMB 5; RMB5; RNA binding motif protein 5; RNA binding protein 5; Tumor suppressor LUCA15; RBM5_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 染色質(zhì)和核信號 信號轉(zhuǎn)導(dǎo) 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Rabbit,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 92 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RBM5/LUCA15: 731-815/815 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
RBM5 (RNA binding motif protein 5) is a nuclear SR related RNA binding protein which specifically binds poly(G) RNA homopolymers in vitro. It modulates both apoptosis and the cell cycle, and retards tumor formation. Subunit: Component of the spliceosome A complex (also known as the prespliceosome). Appears to dissociate from the spliceosome upon formation of the spliceosome B complex (also known as the precatalytic spliceosome), in which the heterotrimeric U4/U6.U5 snRNPs are bound. Interacts with U2AF2; this interaction is direct. Also interacts with ACIN1, PRPF8, SFRS3, SNRPB, SNRPN, SNRNP70 and SNRNP200; these interactions may be indirect. Subcellular Location: Nuclear. Tissue Specificity: Isoform 5 is widely expressed in normal tissues and is expressed at increased levels in T-leukemic cell lines. Similarity: Belongs to the RBM5/RBM10 family. Contains 1 C2H2-type zinc finger. Contains 1 G-patch domain. Contains 1 RanBP2-type zinc finger. Contains 2 RRM (RNA recognition motif) domains. SWISS: P52756 Gene ID: 10181 Database links: Entrez Gene: 10181 Human Entrez Gene: 83486 Mouse Omim: 606884 Human SwissProt: P52756 Human SwissProt: Q91YE7 Mouse Unigene: 439480 Human Unigene: 259197 Mouse Unigene: 17033 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LUCA15) Polyclonal Antibody, Unconjugated (bs-6090R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
