| 產(chǎn)品編號(hào) | bs-3422R |
| 英文名稱 | Phospho-TCTP (Ser46) Rabbit pAb |
| 中文名稱 | 磷酸化翻譯控制腫瘤蛋白抗體 |
| 別 名 | TCTP(phospho Ser46); TCTP(phospho S46); Fortilin; Histamine-releasing factor; p02; p23; TPT1; translationally controlled tumor protein; tumor protein translationally-controlled 1; TCTP_HUMAN. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Cow,Dog,GuineaPig,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 20 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human TCTP around the phosphorylation site of Ser46: DD(p-S)LI |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
TCTP is involved in calcium binding and microtubule stabilization. It is an immunoglobulin E dependent histamine-releasing factor produced by lymphocytes of atopic children and present in biologic fluids of allergic patients. Both human and mouse recombinant HRF proteins caused histamine release from human basophils of a subpopulation of donors (referenced from OMIM). Function: Involved in calcium binding and microtubule stabilization. Subunit: Interacts with STEAP3. Subcellular Location: Cytoplasm. Tissue Specificity: Found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC). Expressed in placenta and prostate. Similarity: Belongs to the TCTP family. SWISS: P13693 Gene ID: 7178 Database links: Entrez Gene: 7178 Human Entrez Gene: 22070 Mouse Omim: 600763 Human SwissProt: P13693 Human SwissProt: P63028 Mouse Unigene: 374596 Human Unigene: 296922 Mouse Unigene: 297482 Mouse Unigene: 36610 Rat 翻譯控制腫瘤蛋白最初發(fā)現(xiàn)于腫瘤組織，后來(lái)又從其他組織中相繼分離出來(lái),是許多動(dòng)物腫瘤細(xì)胞中存在的一類較豐富的蛋白,TCTP參與鈣離子結(jié)合和微管的穩(wěn)定,是一類廣泛存在于動(dòng)物、植物及酵母中的、在序列上高度保守且具有很高表達(dá)量的蛋白家族。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TCTP (Ser46)) Polyclonal Antibody, Unconjugated (bs-3422R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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