| 產(chǎn)品編號(hào) | bs-2723R |
| 英文名稱 | TREM2 Rabbit pAb |
| 中文名稱 | 髓系細(xì)胞觸發(fā)受體2抗體 |
| 別 名 | Triggering receptor expressed on myeloid cells 2; TREM-2; TREM2a; TREM2b; TREM2c; Trggering receptor expressed on myeloid cells 2a; TREM2_HUMAN. |
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Specific References (5) | bs-2723R has been referenced in 5 publications.
[IF=7.561] Elizabeth Olmsted-Davis. et al. A Population of M2 Macrophages Associated With Bone Formation. Front Immunol. 2021; 12: 686769 FC ; Mouse.
[IF=6.986] Dai Shi. et al. Synthesis and Evaluation of 68Ga-NOTA-COG1410 Targeting to TREM2 of TAMs as a Specific PET Probe for Digestive Tumor Diagnosis. Anal Chem. 2022;94(9):3819–3830 WB,IF,IHC ; Mouse,Human.
[IF=4.39] Satoh, Jun-ichi, et al. "A survey of TREM2 antibodies reveals neuronal but not microglial staining in formalin-fixed paraffin-embedded postmortem Alzheimer's brain tissues." Alzheimer's research & therapy 5.4 (2013): 30. WB ; Human.
[IF=4.12] Rai et al. Vitamin D attenuates inflammation, fatty infiltration, and cartilage loss in the knee of hyperlipidemic microswine. (2016) Arthritis.Res.The. 18:203 IF ; Pig.
[IF=1.936] Li et al. TREM2 inhibits inflammatory responses in mouse microglia by suppressing the PI3K/NF-κB signaling. (2018) Cell.Biol.Int. Apr 16. IF&WB ;
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| 研究領(lǐng)域 | 免疫學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 25 kDa |
| 檢測(cè)分子量 | 35-40 |
| 細(xì)胞定位 | 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TREM2 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012] Function: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Subunit: Interacts with TYROBP/DAP12. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein (Potential). Isoform 2: Secreted (Potential). Isoform 3: Secreted (Potential). Tissue Specificity: Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord. DISEASE: Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q9NZC2 Gene ID: 54209 Database links: Entrez Gene: 54209 Human Entrez Gene: 83433 Mouse Omim: 605086 Human SwissProt: Q9NZC2 Human SwissProt: Q99NH8 Mouse Unigene: 435295 Human Unigene: 261623 Mouse TREM-2是近年來(lái)發(fā)現(xiàn)應(yīng)用于診斷炎癥性疾病的重要標(biāo)記物,也是感染性疾病的預(yù)警指標(biāo),與細(xì)菌感染的嚴(yán)重程度相關(guān)。 TREM2屬于免疫球蛋白超家族TREM,表達(dá)于白細(xì)胞的細(xì)胞膜表面受體,TREM-選擇性地表達(dá)于中性粒細(xì)胞和部分單核細(xì)胞,它介導(dǎo)的信號(hào)傳導(dǎo)通路在炎癥的發(fā)生和級(jí)聯(lián)放大中起重要作用。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Raw264.7 cell lysates
Lane 2: Mouse Spleen tissue lysates
Lane 3: Mouse Cerebrum tissue lysates
Lane 4: Mouse BV2 cell lysates
Lane 5: Mouse Lung tissue lysates
Lane 6: Rat Spleen tissue lysates
Lane 7: Rat Cerebrum tissue lysates
Primary: Anti-TREM2 (bs-2723R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 24 kDa
Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TREM2 Polyclonal Antibody, Unconjugated (bs-2723R) at 1:150 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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