| 產(chǎn)品編號 | bs-1514R |
| 英文名稱 | Rabbit Anti-CD33 antibody |
| 中文名稱 | CD33抗體 |
| 別 名 | CD33_HUMAN; CD 33; CD33 antigen; CD33 molecule; FLJ00391; gp67 ; Myeloid cell surface antigen CD33; Myeloid cell surface antigen CD33 precursor; p67 ; Sialic acid binding Ig like lectin 3; Sialic acid binding immunoglobulin like lectin 3; SIGLEC3; Siglec-3; myeloid cell surface antigen CD33 isoform 1 precursor. |
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Specific References (5) | bs-1514R has been referenced in 5 publications.
[IF=5.97] Jiang et al. Involvement of p38 in signal switching from autophagy to apoptosis via the PERK/eIF2α/ATF4 axis in selenite-treated NB4 cells. (2014) Cell.Death.Dis. 5:e1270 IHC ; Mouse.
[IF=4.46] Shi, Kejian, et al. "Sodium selenite alters microtubule assembly and induces apoptosis in vitro and in vivo." Journal of hematology & oncology 6.1 (2013): 1-9. WB ; Human.
[IF=4.24] Shan, Wulin, et al. "LukS-PV, a component of Panton-Valentine leukocidin, exerts potent activity against acute myeloid leukemia in vitro and in vivo." The International Journal of Biochemistry & Cell Biology (2015). IHC-P ; Mouse.
[IF=3.057] Katarzyna Sikorskaet al. The Impact of Ag Nanoparticles and CdTe Quantum Dots on Expression and Function of Receptors Involved in Amyloid-β Uptake by BV-2 Microglial Cells. Materials (Basel)
. 2020 Jul 20;13(14):3227. FCM ; mouse.
[IF=2.936] Han Y et al. Chemical metabolomics for investigating the protective effectiveness of Acanthopanax senticosus Harms leaf against acute promyelocytic leukemia.RSC Adv., 2018, 8, 11983. IHC ; Mouse.
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| 研究領(lǐng)域 | 免疫學(xué) 干細胞 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 檢測分子量 | 67 |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse CD33: 101-200/334 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome. [provided by Alliance of Genome Resources, Apr 2022] Function: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Subunit: Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Monocytic/myeloid lineage cells. Post-translational modifications: Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. Similarity: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q63994 Gene ID: 12489 Database links: Entrez Gene: 945 Human Entrez Gene: 12489 Mouse Omim: 159590 Human SwissProt: P20138 Human SwissProt: Q63994 Mouse Unigene: 83731 Human Unigene: 140157 Mouse
CD33(Siglec-3)也是急性淋巴細胞白血病抗原,細胞表面跨膜糖蛋白.CD33表達于早期B淋巴細胞,某些粒性白細胞,骨髓基質(zhì)細胞,部分上皮組織及其起源的腫瘤細胞中也有表達.主要應(yīng)用于某些惡性淋巴瘤和白血病的分型. |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Bone (Mouse) Lysate at 40 ug
Lane 2: Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-CD33 (bs-1514R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40/37 kD
Observed band size: 65 kD
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