| 產(chǎn)品編號 | bs-13356R-BF488 |
| 英文名稱 | Rabbit Anti-Giantin/BF488 Conjugated antibody |
| 中文名稱 | BF488標記的高爾基體相關(guān)蛋白GM130抗體 |
| 別 名 | 372 kDa Golgi complex associated protein; 372 kDa Golgi complex-associated protein; GCP; GCP372; Giantin; GOGB1_HUMAN; GOLGB1; Golgi autoantigen golgin subfamily a member 1; Golgi autoantigen golgin subfamily b macrogolgin (with transmembrane signal) 1; Golgi autoantigen, golgin subfamily b, macrogolgin 1; Golgi complex associated protein 372kDa; Golgi complex associated protein 372kDa; Golgi complex-associated protein 372kDa; Golgi complex-associated protein 372kDa; Golgin B1; Golgin B1, golgi integral membrane protein; Golgin subfamily B member 1; GOLIM1; Macrogolgin; Macrogolgin; MGC33154. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導 細胞類型標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Dog, Horse, Rabbit, Hamster, Monkey, ) |
| 產(chǎn)品應用 | Flow-Cyt=1:20-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 376kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Giantin/GM130 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Feb 2010] Function: May participate in forming intercisternal cross-bridges of the Golgi complex. Subunit: Homodimer; disulfide-linked. Interacts with PLK3. Subcellular Location: Golgi apparatus membrane. Database links: Entrez Gene: 2801 Human Entrez Gene: 99412 Mouse Omim: 602580 Human SwissProt: Q08379 Human SwissProt: Q921M4 Mouse Unigene: 155827 Human Unigene: 106376 Mouse Unigene: 6165 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |
Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-Giantin antibody (bs-13356R-BF488)
Dilution: 1ug/Test;
Protocol
The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
