| 產(chǎn)品編號 | bs-10045R-HRP |
| 英文名稱 | Rabbit Anti-CSFV/HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標記的豬瘟病毒抗體 |
| 別 名 | Polyprotein [Classical swine fever virus,CSFV] |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 免疫學(xué) 細菌及病毒 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | |
| 產(chǎn)品應(yīng)用 | ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Classical swine fever virus |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Classical swine fever(CSF) is a kind of acute and intense contagious disease in swine, it material damages swine industry. CSF could cause swine appearing disseminated hemorrhage in systemic organ and tissue, degeneration and necrosis in small vascular and capillary endothelial cells. Classical swine fever virus (CSFV) belongs to genera Pestivirus, family Flaviviridae, it’s genome total length is 12.3Kb,including a big ORF, encoding 11 structural and nonstructural proteins. Database links: Entrez Gene: 920146 CSFVA SwissProt: P19712 CSFVA Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 豬瘟(Classical Swine Fever,CSF)是由豬瘟病毒(CSFV)引起的豬急性高度接觸性傳染性疾病,是嚴重威脅養(yǎng)豬業(yè),具有重要經(jīng)濟意義的病毒性疾病之一,豬瘟可引起豬的全身組織器官彌漫性出血,小血管和毛細血管內(nèi)皮細胞發(fā)生變性、壞死。 豬瘟病毒為黃病毒科(Flaviviridae)瘟病毒屬(Pestivirus)成員,基因組大小約12.3kb,含一個大的開放閱讀框(ORF),由11 個編碼結(jié)構(gòu)蛋白與非結(jié)構(gòu)蛋白的基因組成。豬瘟病毒對血管組織具有親嗜性,病理組織學(xué)檢查時發(fā)現(xiàn),組織器官的出血斑點主要是由于小血管和毛細血管內(nèi)皮細胞發(fā)生腫脹、變性和壞死;梗死灶的發(fā)生主要是由于小動脈管內(nèi)皮細胞發(fā)生變性、壞死、剝脫后,血管內(nèi)膜粗糙,官腔內(nèi)血栓形成,導(dǎo)致官腔狹窄或閉塞所致。此外,還可見到心肌呈實質(zhì)變性,但病豬的腎臟等器官的實質(zhì)細胞沒有變性、壞死等病理變化。 |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
