| 產(chǎn)品編號 | bs-12011R-BF647 |
| 英文名稱1 | Rabbit Anti-GRM1/BF647 Conjugated antibody |
| 中文名稱 | BF647標記的代謝型谷氨酸受體1A抗體 |
| 別 名 | G protein coupled receptor family C group 1 member A; Glutamate receptor metabotropic 1 alpha; Glutamate receptor metabotropic 1; GPRC1A; GRM 1; GRM1 alpha; GRM1; GRM1A; Human metabotropic glutamate receptor 1 alpha mRNA complete cds; Metabotropic glutamate receptor 1 alpha; Metabotropic glutamate receptor 1; mGlu1; MGLUR1; MGLUR1A; mGluR1alpha; metabotropic glutamate receptor 1 isoform alpha; GRM1_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細胞生物 神經(jīng)生物學 細胞膜受體 G蛋白偶聯(lián)受體 新陳代謝 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, ,Danio rerio) |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=1ug/Test ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 130kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MGLUR1A/Metabotropic Glutamate Receptor 1a |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: This gene encodes a metabotropic glutamate receptor that functions by activating phospholipase C. L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The canonical alpha isoform of the encoded protein is a disulfide-linked homodimer whose activity is mediated by a G-protein-coupled phosphatidylinositol-calcium second messenger system. This gene may be associated with many disease states, including schizophrenia, bipolar disorder, depression, and breast cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013] Function: Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. Subunit: Homodimer; disulfide-linked. The PPXXF motif binds HOMER1, HOMER2 and HOMER3. Interacts with SIAH1, RYR1, RYR2, ITPR1, SHANK1, SHANK3 and GRASP Subcellular Location: Cell Membrane; multi pass membrane protein. Similarity: Belongs to the G-protein coupled receptor 3 family. Database links: Entrez Gene: 2911 Human Entrez Gene: 14816 Mouse Omim: 604473 Human SwissProt: Q13255 Human SwissProt: P97772 Mouse Unigene: 32945 Human Unigene: 391904 Mouse Unigene: 482986 Mouse Unigene: 87787 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-GRM1 antibody (bs-12011R-BF647)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
