| 產(chǎn)品編號 | bs-3640R-BF488 |
| 英文名稱 | Rabbit Anti-Plectin/BF488 Conjugated antibody |
| 中文名稱 | BF488標(biāo)記的網(wǎng)蛋白抗體 |
| 別 名 | EBS1; EBSO; HD1; Hemidesmosomal protein 1; PCN; PLEC1; PLEC1b; Plectin 1 plectin 1 intermediate filament binding protein 500kDa; Plectin 6; PLTN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human, Mouse, Rat, Dog, Cow, Horse, ) |
| 產(chǎn)品應(yīng)用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 515kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Plectin |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Plectin interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the crosslinking and stabilization of cytoskeletal intermediate filaments network, but also in the regulation of their dynamics. Function: Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofibers integrity. Subunit: Homodimer or homotetramer. Interacts (via actin-binding domain) with Nesprin-3. Interacts (via CH 1 domain) with VIM (via rod region). Interacts with FER (By similarity). Interacts with COL17A1 (via N-terminus). Interacts (via N-terminus) with DST isoform 1 (via N-terminus). Subcellular Location: Cytoplasm, cytoskeleton. Cell junction, hemidesmosome. Tissue Specificity: Widely expressed with highest levels in muscle, heart, placenta and spinal cord. Post-translational modifications: Phosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis. Phosphorylated upon DNA damage, probably by ATM or ATR. DISEASE: Defects in PLEC are the cause of epidermolysis bullosa simplex with pyloric atresia (EBS-PA) [MIM:612138]. EBS-PA is an autosomal recessive genodermatosis characterized by severe skin blistering at birth and congenital pyloric atresia. Death usually occurs in infancy. This disorder is allelic to MD-EBS. Similarity: Belongs to the plakin or cytolinker family. Contains 1 actin-binding domain. Contains 2 CH (calponin-homology) domains. Contains 33 plectin repeats. Contains 4 spectrin repeats. Database links: Entrez Gene: 5339 Human Entrez Gene: 18810 Mouse Omim: 601282 Human SwissProt: Q15149 Human SwissProt: Q9QXS1 Mouse Unigene: 434248 Human Unigene: 234912 Mouse Unigene: 1085 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 網(wǎng)蛋白(plectin)在介導(dǎo)、維持、穩(wěn)定細胞骨架中起到關(guān)鍵作用，在微管、微絲和中間絲之間的連接中具有結(jié)構(gòu)性功能。 |
