| 產(chǎn)品編號(hào) | bs-3099R-AP |
| 英文名稱(chēng) | Rabbit Anti-Phospho-Cofilin (Ser3)/AP Conjugated antibody |
| 中文名稱(chēng) | 堿性磷酸酶（AP）標(biāo)記的磷酸化絲切蛋白(Ser3)抗體 |
| 別 名 | Cofilin (Phospho-Ser3); Cofilin (Phospho S3); 18 kDa phosphoprotein; CFL 1; CFL; CFL1; Cofilin 1; Cofilin 1 non muscle; Cofilin non muscle isoform; p18; COF1_HUMAN. |
| 規(guī)格價(jià)格 | 100ul/2980元 購(gòu)買(mǎi) 大包裝/詢(xún)價(jià) |
| 說(shuō) 明 書(shū) | 100ul |
| 產(chǎn)品類(lèi)型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 生長(zhǎng)因子和激素 細(xì)胞粘附分子 細(xì)胞類(lèi)型標(biāo)志物 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Mouse, Rat, (predicted: Human, Pig, Cow, Sheep, ) |
| 產(chǎn)品應(yīng)用 | WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 18kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Cofilin around the phosphorylation site of Ser3 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]. Function: Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Subunit: Can bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is a major component of intranuclear and cytoplasmic actin rods. Subcellular Location: Nucleus matrix. Cytoplasm, cytoskeleton. Tissue Specificity: Widely distributed in various tissues. Post-translational modifications: Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells. Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal. Similarity: Belongs to the actin-binding proteins ADF family. Contains 1 ADF-H domain. Database links: Entrez Gene: 1072 Human Entrez Gene: 12631 Mouse Entrez Gene: 494432 Sheep Omim: 601442 Human SwissProt: P23528 Human SwissProt: P18760 Mouse SwissProt: Q6B7M7 Sheep Unigene: 52428 Cow Unigene: 170622 Human Unigene: 329655 Mouse Unigene: 11675 Rat Unigene: 13212 Sheep Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 絲切蛋白(cofilin)是細(xì)胞骨架塑形的重要調(diào)節(jié)因素,其主要功能是分解肌動(dòng)蛋白微絲和增加肌動(dòng)蛋白單體從肌動(dòng)蛋白微絲的末端解離的速度,從而促進(jìn)肌動(dòng)蛋白微絲的循環(huán),是目前為止已發(fā)現(xiàn)的肌動(dòng)蛋白結(jié)合蛋白家族中唯一能夠改變肌動(dòng)蛋白微絲扭曲結(jié)構(gòu)的蛋白. |
