| 產(chǎn)品編號 | bs-10057R-HRP |
| 英文名稱 | Rabbit Anti-PCV2 capsid protein/HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標記的豬圓環(huán)病毒Ⅱ型衣殼蛋白抗體 |
| 別 名 | ORF1; ORF2 (PCV-2; PCV 2; Porcine circovirus2; Porcine circovirus-2.) |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 細菌及病毒 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | |
| 產(chǎn)品應(yīng)用 | ELISA=1:100-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 28kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from PCV2 capsid protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: There has been much uncertainty in the relationship between PCV2 and PMWS but Bolin et al demonstrated that Koch's postulates could be fulfilled by inoculating cesarean-derived, colostrum-deprived (CDCD) pigs with PCV2 and producing clinical signs of PMWS. Similar work has been performed using an infectious genomic clone of PCV2 in specific pathogen free (SPF) pigs to produce the gross and microscopic lymphoid lesions of PMWS. Although PCV2 has been shown to induce PMWS lesions in CDCD and SPF pigs, the presence of additional factors are required. Function: Self-assembles to form the virion icosahedral capsid with a T=1 symmetry. This very small capsid (17 - 22 nm in diameter) allows the virus to be very stable in the environment and resistant to some disinfectants, including detergents. Essential for the initial attachment to heparan sulfate moities and chondroitin sulfate B of the host cell surface proteoglycans. After attachment, the virus is internalized in a clathrin-, caveolae- and dynamin-independent, actin and Rho-GTPase-mediated pathway and traffics to the nucleus. The capsid protein binds and transports the viral genome and Rep across the nuclear envelope. Subunit: Homomultimer. Assembles in the nucleus, presumably in an immature form, then migrates to the cytoplasm once assembled as mature virion (Probable). Interacts with Rep; this interaction relocates Rep into the nucleus. Subcellular Location: Host nucleus. Virion (Potential). Similarity: Belongs to the circoviridae capsid protein family. Database links: Entrez Gene: 2943185 PCV2 SwissProt: O56129 PCV2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 豬圓環(huán)病毒-PCV-2是一種嗜單核-巨噬細胞性的病毒，屬于圓環(huán)病毒科圓環(huán)病毒屬的成員，根據(jù)抗原性及基因組成，分為PCVl和PCV2兩種，其中PCV2對豬具有致病性，PCV2(病毒囊膜衣殼蛋白(Cap))主要侵害免疫系統(tǒng)，導(dǎo)致感染豬免疫抑制，降低機體對其他病原體的抵抗力和疫苗的反應(yīng)性，容易導(dǎo)致其他病毒和細菌的繼發(fā)感染，使死亡率明顯升高。 |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
