| 產(chǎn)品編號(hào) | bs-1970R-BF488 |
| 英文名稱 | Rabbit Anti-LAMP1/BF488 Conjugated antibody |
| 中文名稱 | BF488標(biāo)記的溶酶體相關(guān)膜蛋白1抗體 |
| 別 名 | CD107 antigen like family member A; CD107 antigen-like family member A; CD107a; CD107a antigen; LAMP 1; LAMP-1; LAMP1_HUMAN; LAMPA; LGP120; lgpA; Lysosomal membrane glycoprotein 120KD; Lysosomal Associated Membrane Protein 1; Lysosome associated membrane glycoprotein 1; Lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1; OTTHUMP00000040663. |
| 規(guī)格價(jià)格 | 100ul/2980元 購買 大包裝/詢價(jià) |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 干細(xì)胞 細(xì)胞類型標(biāo)志物 新陳代謝 細(xì)胞膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=2ug/Test IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 42kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LAMP-1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation. Function: Presents carbohydrate ligands to selectins. Also mplicated in tumor cell metastasis. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane. Post-translational modifications: O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. Similarity: Belongs to the LAMP family. Database links: Entrez Gene: 3916 Human Entrez Gene: 16783 Mouse Omim: 153330 Human SwissProt: P11279 Human SwissProt: P11438 Mouse Unigene: 494419 Human Unigene: 16716 Mouse Unigene: 475822 Mouse Unigene: 40177 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CD107a可很好的反映NK細(xì)胞活性，其表達(dá)水平的上調(diào)與NK分泌細(xì)胞因子的增多及NK對(duì)靶細(xì)胞的殺傷作用的增強(qiáng)高度相關(guān)，采用流式檢測(cè)，CD107a還可鑒定出一群無細(xì)胞因子分泌的活性NK細(xì)胞。 |
| 產(chǎn)品圖片 |
Blank control:MCF7.
Primary Antibody (green line): Rabbit Anti-LAMP1 antibody (bs-1970R-BF488)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:A431.
Primary Antibody (green line): Rabbit Anti-LAMP1 antibody (bs-1970R-BF488)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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