| 產(chǎn)品編號 | bs-1537R-Bio |
| 英文名稱 | Rabbit Anti-NADPH/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的還原型輔酶Ⅱ/磷酸酰胺腺嘌呤二核苷酸抗體 |
| 別 名 | nicotinamide adenine dinucleotide phosphate; NADP; NADPH; TPNH; Triphosphopyridine nucleotide, reduced form; |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 產(chǎn)品類型 | 小分子抗體 |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | |
| 產(chǎn)品應(yīng)用 | ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 0.833kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated NADPH |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: NDAPH （nicotinamide adenine dinucleotide phosphate) includes of Alpha-NADPH and Beta-NADPH. Beta-NADPH hase activity only. Beta-Nicotinamide adenine dinucleotide 2'-phosphate reduced tetrasodium salt or triphosphopyridine nucleotide reduced tetrasodium salt, also known as Coenzyme Ⅱ. β-Nicotinamide adenine dinucleotide 2′-phosphate (NADP+) and β-Nicotinamide adenine dinucleotide 2′-phosphate, reduced (NADPH) comprise a coenzyme redox pair (NADP+:NADPH) involved in a wide range of enzyme catalyzed oxidation reduction reactions. The NADP+/NADPH redox pair facilitates electron transfer in anabolic reactions such as lipid and cholesterol biosynthesis and fatty acyl chain elongation. The NADP+/NADPH redox pair is used in a variety of antioxidation mechanism where it protects agains reactive oxidation species accumulation. NADPH is generated in vivio by the pentose phosphate pathway (PPP). Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. NADPH氧化酶廣泛存在于機體多個系統(tǒng)發(fā)揮重要的生理功能。在多種病理情況下，NADPH氧化酶可以在很多組織中催化合成大量活性氧，導(dǎo)致過度氧化應(yīng)激，使組織細胞的功能下降。 NADPH氧化酶也是血管內(nèi)皮細胞ROS產(chǎn)生的主要酶系,由其介導(dǎo)產(chǎn)生ROS在信息傳遞、基因轉(zhuǎn)錄、細胞生長和凋亡以及調(diào)節(jié)內(nèi)皮依賴性的一氧化氮合酶脫偶聯(lián)等血管舒張中的作用,使其成為血管生物學(xué)中重要的部分,NADPH氧化酶一方面與血管舒張的機能異常有關(guān)，另一方面又影響到內(nèi)皮細胞的結(jié)構(gòu)、粘附分子的表達、滲透、成長及遷移等功能。 |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
