| 產(chǎn)品編號 | bs-42105R |
| 英文名稱 | Complement C3d fragment Rabbit pAb |
| 中文名稱 | 補體C3d片段抗體 |
| 別 名 | Acylation stimulating protein; ASP; C3; C3a anaphylatoxin; Complement C3 alpha chain; Complement C3; Complement C3 precursor; Complement component 3; Complement component C3; Complement factor 3; Plp; Complement C3d fragment; CO3_HUMAN. |
| 研究領(lǐng)域 | 細胞生物 免疫學 信號轉(zhuǎn)導 G蛋白偶聯(lián)受體 淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 33/109/187 kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Complement C3d fragment protein: 1002-1663/1663 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes. SWISS: P01024 Gene ID: 718 Database links: Entrez Gene: 718 Human Omim: 120700 Human SwissProt: P01024 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human U87MG cell lysates
Lane 3: Human A431 cell lysates
Lane 4: Human HeLa cell lysates
Lane 5: Human A549 cell lysates
Primary: Anti-Complement C3d fragment (bs-42105R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33/109/187 kDa
Observed band size: 75 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
