| 產(chǎn)品編號 | bs-41387R |
| 英文名稱 | PRRSV N Rabbit pAb |
| 中文名稱 | 豬藍耳病病毒N蛋白抗體 |
| 別 名 | PRRSV N protein; N protein [Porcine reproductive and respiratory syndrome virus]; nucleocapsid protein; |
| 研究領域 | 細菌及病毒 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Pig,PRRSV |
| 產(chǎn)品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 13.8 kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant PRRSV N protein: 1-128/128 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
PRRSV is a small, enveloped RNA virus. It contains a single-stranded, positive-sense, RNA genome with a size of approximately 15 kilobases. The genome contains nine open reading frames. PRRSV is a member of the genus Arterivirus, family Arteriviridae, order Nidovirales. Subclinical infections are common, with clinical signs occurring sporadically in a herd. Clinical signs include reproductive failure in sows such as abortions and giving birth to stillborn or mummified fetuses, and cyanosis of the ear and vulva. In neonatal pigs, the disease causes respiratory distress, with increased susceptibility to respiratory infections such as Glasser's disease. |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Recombinant PRRSV N protein, N-His(bs-41387P)
Primary: Anti-PRRSV N (bs-41387R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13.8 kDa
Observed band size: 17 kDa
Measured by its binding ability in a indirect ELISA. Immobilized Pig PRRSV?protein, His Tag (Cat. bs-41387P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Pig PRRSV?Antibody, the EC50 is 400.8 ng/mL.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
