| 產(chǎn)品編號 | bsm-30304M |
| 英文名稱 | human CD30 Mouse mAb |
| 中文名稱 | 人CD30單克隆抗體 |
| 別 名 | CD 30; CD30 antigen; CD30L receptor; Cytokine receptor CD30; D1S166E; KI 1; Ki 1 antigen; Ki-1 antigen; KI1; Lymphocyte activation antigen CD30; TNFRSF 8; TNFRSF8; TNR8_HUMAN; Tumor Necrosis Factor Receptor Superfamily Member 8; Tumor Necrosis Factor Rece |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 3C11 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 62 kDa |
| 檢測分子量 | 105 |
| 性 狀 | Liquid |
| 亞 型 | Mouse IgG1, k |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4). |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| 產(chǎn)品圖片 |
Blank control（black line）:SR.
Primary Antibody (green line): Mouse Anti-human CD30 antibody (bsm-30304M)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-mouse IgG-FITC
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Mouse IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
