| 產品編號 | bsm-60543R |
| 英文名稱 | Rabbit Anti-PHB2 antibody |
| 中文名稱 | B細胞受體相關蛋白重組兔單抗 |
| 別 名 | PHB2_HUMAN; Prohibitin-2; B-cell receptor-associated protein BAP37; D-prohibitin; Repressor of estrogen receptor activity; BAP; REA; p22; hBAP; Bap37; BCAP37; PNAS-141; |
| 研究領域 | 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | F5G8 |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產品應用 | WB=1:2000-1:10000,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 細胞核 細胞漿 細胞膜 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG/Kappa |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Enables several functions, including protein C-terminus binding activity; protein N-terminus binding activity; and protein dimerization activity. Involved in several processes, including defense response to virus; positive regulation of cell cycle phase transition; and regulation of transcription, DNA-templated. Located in several cellular components, including cell surface; mitochondrial membrane; and nuclear matrix. Part of mitochondrial prohibitin complex. [provided by Alliance of Genome Resources, Apr 2022] Subcellular Location: Mitochondrion inner membrane. Cytoplasm. Nucleus. Also cytoplasmic and nuclear. SWISS: Q99623 Gene ID: 11331 Database links: Entrez Gene: 11331 Human Entrez Gene: 12034 Mouse SwissProt: Q99623 Human SwissProt: O35129 Mouse |
| 產品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:10000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: HeLa, 293, Mouse heart, Rat kidney
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 33 kDa
Observed MW: 33 kDa
Cell line: HeLa
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60543R
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
