| 產品編號 | bs-42003R |
| 英文名稱 | alpha Internexin Rabbit pAb |
| 中文名稱 | α-中連蛋白抗體 |
| 別 名 | a-Internexin; alpha-Internexin; alpha-Internexin; 66 kDa neurofilament protein; alpha Inx; INA; Internexin neuronal intermediate filament protein alpha; MGC12702; NEF 5; NEF5; Neurofilament 5(66kD); Neurofilament 66; Neurofilament 66 tax binding protein; |
| 研究領域 | 神經生物學 干細胞 細胞粘附分子 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 55 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Alpha-internexin protein: 1-499/499 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
eurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons. [provided by RefSeq, Jun 2009] SWISS: Q16352 Gene ID: 9118 Database links: Entrez Gene: 9118 Human Entrez Gene: 226180 Mouse Omim: 605338 Human SwissProt: Q16352 Human SwissProt: P46660 Mouse Unigene: 500916 Human Unigene: 276251 Mouse Unigene: 88941 Rat |
| 產品圖片 |
Sample:
Lane 1: Human SH-SY5Y cell lysates
Lane 2: Human SH-SY5Y cell lysates
Primary: Anti-alpha Internexin (bs-42003R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 66 kDa
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Primary: Anti-alpha Internexin (bs-42003R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 60 kDa
Measured by its binding ability in a indirect ELISA. Immobilized Human alpha Internexin protein, His Tag (Cat. bs-42003P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Human alpha Internexin Antibody, the EC50 is 911.2 ng/mL.
Measured by its binding ability in a indirect ELISA. Immobilized Human alpha Internexin protein, His Tag (Cat. bs-42003P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Human alpha Internexin Antibody, the EC50 is 312.5 ng/mL.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
