| 產(chǎn)品編號 | bsm-30074M |
| 英文名稱 | human CD14 Mouse mAb |
| 中文名稱 | 小鼠抗人CD14單克隆抗體 |
| 別 名 | Monocyte Differentiation Antigen 14; CD14 antigen; CD14 molecule; Lipopolysaccharide receptor; LPSR; Monocyte differentiation antigen CD14; Myeloid cell specific leucine rich glycoprotein; CD14_HUMAN; Myeloid cell-specific leucine-rich glycoprotein. |
| 研究領(lǐng)域 | 細胞生物 免疫學 干細胞 細胞表面分子 細胞類型標志物 淋巴細胞 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 14F4 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | Flow-Cyt=1ul/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 35/40 kDa |
| 檢測分子量 | 53-55 |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | Mouse IgG1, k |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4). |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010] Function: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. Subunit: Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Similarity: Contains 11 LRR (leucine-rich) repeats. Database links: Entrez Gene: 929 Human Entrez Gene: 12475 Mouse Omim: 158120 Human SwissProt: P08571 Human Unigene: 163867 Human CD14又稱內(nèi)毒素受體，屬細胞表面糖蛋白家族成員之一。在機體免疫、防御系統(tǒng)引起的一系列反應中介導細胞識別LPS并引起細胞酪氨酸磷酸化、核因子NF-κB轉(zhuǎn)位、觸發(fā)細胞因子釋放和氧自由基產(chǎn)生。CD14在細胞因子產(chǎn)生的調(diào)控方面起重要作用,因而對于CD14結(jié)構(gòu)、功能及其臨床意義的研究日益受到重視。 |
| 產(chǎn)品圖片 |
Blank control:whole blood.
Primary Antibody (green line): Mouse Anti-CD14 antibody (bsm-30074M)
Dilution: 1ul/Test;
Secondary Antibody : Goat anti-mouse IgG-FITC
Dilution: 0.5ug/Test.
Protocol
Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
