| 產品編號 | bs-43048P |
| 英文名稱 | Recombinant SARS-Cov-2 Spike RBD protein (L452R,T478K), His (HEK293) |
| 中文名稱 | 重組SARS冠狀病毒2 Spike突變蛋白RBD(L452R,T478K) |
| 別 名 | SARS-CoV-2 spike protein; 2019-nCOV Spike protein; Spike glycoprotein; Spike protein S1; Surface glycoprotein; Spike protein RBD; SPIKE_SARS2; 2019-nCOV Spike RBD (L452R,T478K), Recombinant (His Tag); Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD (L452R,T4 新冠病毒S蛋白RBD; 新冠S蛋白RBD; SARS-CoV-2 S蛋白RBD; |
| 理論分子量 | 28.2 kDa |
| 檢測分子量 | 36 |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | ＞0.5 mg/ml |
| 物 種 | SARS-Cov-2 |
| 序 列 | 319-541/1273 |
| 純 度 | >90% as determined by SDS-PAGE |
| 純化方法 | AC |
| 內毒素 | Not analyzed |
| 表達系統(tǒng) | HEK293 cell |
| 活性 | Yes |
| 標簽 | His |
| 緩 沖 液 | PBS (pH7.4). |
| 保存條件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to specific receptors on the host cell. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be necessary for the binding of the virus to the host cell at the advent of the infection process. It has been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor-binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 provides essential elements needed for membrane fusion. The S protein plays vital parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; the Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor-binding domain (RBD), leading to a higher mortality rate.
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| 產品圖片 |
Measured by its binding ability in a functional ELISA. Immobilized human ACE2, His-Avi Tag (Cat: bs-46001P) at 2μg/ml (100ul/Well) can bind SARS-CoV-2 Spike RBD (L452R, T478K), His Tag (Cat: bs-43048P), the EC50 is 41ng/ml.
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
