| 產(chǎn)品編號 | bsm-51728M |
| 英文名稱 | PLIN3 Mouse mAb |
| 中文名稱 | PLIN3單克隆抗體 |
| 別 名 | TIP47; PLIN3_HUMAN; 47 kDa MPR binding protein; Cargo selection protein TIP47; M6PRBP 1; M6PRBP1; Mannose 6 phosphate receptor binding protein 1; MGC11117; MGC2012; MPR binding protein 47 kDa; Perilipin 3; Perilipin3; Perilipin-3; Placental protein 17; PL |
| 研究領域 | 信號轉導 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | D2A3 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-2000,Flow-Cyt=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PLIN3: 1-434/434 |
| 亞 型 | IgG2a,k |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2009] Subunit: Homooligomer. Interacts with M6PR (via the cytoplasmic domain). Interacts with IGF2R (via the cytoplasmic domain). Isoform 2 may exist as a homodimer (known as PP17C). Subcellular Location: Cytoplasm. Endosome membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Lipid droplet (Potential). Note=Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails. Similarity: Belongs to the perilipin family. SWISS: O60664 Gene ID: 10226 Database links: Entrez Gene: 10226 Human SwissProt: O60664 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Hela cell lysates
Lane 2: K562 cell lysates
Lane 3: Daudi cell lysates
Primary: Anti-PLIN3 (bsm-51728M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Blank control: HUVEC.
Primary Antibody (green line): Mouse Anti-PLIN3 antibody (bsm-51728M)
Dilution: 1:25;
Isotype Control Antibody (blue line): Mouse IgG
Secondary Antibody : Goat anti-mouse IgG-AF488
Dilution: 1:200
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
