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Mouse Anti-Prohibitin  antibody (bsm-51570M)  
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產(chǎn)品編號 bsm-51570M
英文名稱 Mouse Anti-Prohibitin  antibody
中文名稱 阻抑素/腫瘤抑制因子單克隆抗體
別    名 Mitochondrial Marker; PHB; PHB1; Prohibitin-Mitochondrial Marker; PHB_HUMAN.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  染色質(zhì)和核信號  細(xì)胞凋亡  轉(zhuǎn)錄調(diào)節(jié)因子  細(xì)胞類型標(biāo)志物  鋅指蛋白  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 E4F12
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-1000,IHC-P=1:25-50,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 30 kDa
檢測分子量
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinant human Prohibitin between 1-205 amino acids. 
亞    型 IgG2b,k
純化方法 affinity purified by Protein G
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Function:
Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.

Subunit:
Interacts with PHB2. Interacts with STOML2.

Subcellular Location:
Mitochondrion inner membrane.

Tissue Specificity:
Widely expressed in different tissues.

Similarity:
Belongs to the prohibitin family.

SWISS:
P35232

Gene ID:
5245

Database links:

Entrez Gene: 5245 Human

SwissProt: P35232 Human



產(chǎn)品圖片
Sample: Lane 1: 293 cell lysates Lane 2: MCF-7 cell lysates Lane 3: HepG2 cell lysates Lane 4: NIH/3T3 cell lysates Lane 5: Rat Liver tissue lysates Primary: Anti-Prohibitin (bsm-51570M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD
Paraformaldehyde-fixed, paraffin embedded (Human stomach section); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Prohibitin) Monoclonal Antibody, Unconjugated (bsm-51570M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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