| 產(chǎn)品編號 | bs-55098R |
| 英文名稱 | Rabbit Anti-HMGB1 antibody |
| 中文名稱 | 高遷移率族蛋白B1抗體 |
| 別 名 | High mobility group protein B1; Amphoterin; High mobility group 1; High Mobility Group Box 1; High mobility group protein 1; HMG3; HMGB 1; HMGB-1; Hmgb1 protein; Nonhistone chromosomal protein HMG1; SBP 1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; HMGB1_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 免疫學 轉(zhuǎn)錄調(diào)節(jié)因子 結(jié)合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-1000,IHC-P=1:50-100,IHC-F=1:400-800,IF=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 25kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HMGB1: 100-200/215 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
High Mobility Group Box-1 (HMGB1) is a cytokine implicated in the pathogenesis of rheumatoid arthritis (RA) and other inflammatory diseases. The cholinergic anti-inflammatory pathway, a vagus nerve dependent mechanism, inhibits HMGB1 release in experimental disease models Function: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Subunit: Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Subcellular Location: Nucleus. Chromosome. Similarity: Belongs to the HMGB family. Contains 2 HMG box DNA-binding domains. SWISS: P09429 Gene ID: 3146 Database links: Entrez Gene: 3146 Human Entrez Gene: 100862258 Mouse Entrez Gene: 15289 Mouse Omim: 163905 Human SwissProt: P09429 Human 近來的研究表明稱之為高遷移率族蛋白B-1（HMG-B1）的核內(nèi)結(jié)構蛋白在核外表達時是一種有效的早期炎癥介質(zhì)。 高遷移性Ｂ１組蛋白（ＨＭＧＢ１）： 是一種核結(jié)合蛋白，在ＤＮＡ重組、修復、復制和基因轉(zhuǎn)錄中起作用。ＨＭＧＢ１也是巨噬細胞分泌的一種介質(zhì)。 此外，高遷移性Ｂ１組蛋白亦被受刺激的巨噬細胞或單核細胞主動分泌。在這一主動分泌過程中，HMGB1首先經(jīng)乙酰化并由核內(nèi)轉(zhuǎn)移至溶酶體內(nèi)，繼而在ATP和溶血磷脂膽堿兩種分泌信號指導下轉(zhuǎn)移至細胞外。由壞死細胞被動釋放的HMGB1和炎癥細胞主動分泌的HMGB1存在分子上的差異。胞外的HMGB1可作為細胞因子參與信號傳導，因為它既可識別Toll 樣受體（TLR）家族的一些成員，又能與識別晚期糖基化終末產(chǎn)物受體（RAGE）相作用。 HMGB1能啟動炎癥反應，包括產(chǎn)生多種細胞因子、對某些干細胞產(chǎn)生趨化作用、誘導血管粘附分子、削弱腸上皮細胞的功能等 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human Hela Cell (Control) Lysate
Lane 2: HMGB1 knockout (KO) HeLa Cell Lysate
Primary: Anti-HMGB1 (bs-55098R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 30 kD
Sample:
Lane 1: Lung (Mouse) TissueLysate at 40 ug
Lane 2: Liver (Mouse) Tissue Lysate at 40 ug
Lane 3: Testis (Rat) Tissue Lysate at 40 ug
Lane 4: Cerebrum (Rat) Tissue Lysate at 40 ug
Lane 5: Jurkat (Human) Cell Lysate at 30 ug
Lane 6: Hela (Human) Cell Lysate at 30 ug
Lane 7: HepG2 (Human) Cell Lysate at 30 ug
Lane 8: Molt-4 (Human) Cell Lysate at 30 ug
Primary:
Anti-HMGB1 (bs-55098R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (bs-55098R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (bs-55098R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (bs-55098R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
