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Rabbit Anti-MEKK3  antibody (bsm-52709R)  
~~~促銷,代碼KT202504~~~
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說明書: 50ul  100ul  
50ul/1400.00元
100ul/2500.00元
大包裝/詢價

產(chǎn)品編號 bsm-52709R
英文名稱 Rabbit Anti-MEKK3  antibody
中文名稱 MAPK/ERK激酶3重組兔單抗
別    名 M3K3_HUMAN; MAP/ERK kinase kinase 3; MAP3K3; MAPK/ERK kinase kinase 3; MAPKKK3; MEK kinase 3; MEKK 3; MEKK3; mitogen activated protein kinase kinase kinase 3; Mitogen-activated protein kinase kinase kinase 3.  
研究領(lǐng)域 細(xì)胞生物  信號轉(zhuǎn)導(dǎo)  激酶和磷酸酶  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 5C1
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:50-100,ICC/IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 71 kDa
檢測分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MEKK3 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Function:
Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.

Similarity:
Belongs to the protein kinase superfamily.
STE Ser/Thr protein kinase family.
MAP kinase kinase kinase subfamily.
Contains 1 OPR domain.
Contains 1 protein kinase domain.

SWISS:
Q99759

Gene ID:
4215

Database links:

Entrez Gene: 4215 Human

Entrez Gene: 26406 Mouse

Entrez Gene: 303604 Rat

Omim: 602539 Human

SwissProt: Q99759 Human

SwissProt: Q61084 Mouse

Unigene: 29282 Human

Unigene: 27041 Mouse

Unigene: 72680 Rat



產(chǎn)品圖片
25 ug total protein per lane of various lysates (see on figure) probed with MEKK3 monoclonal antibody, unconjugated (bsm-52709R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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