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AGR2 Recombinant Rabbit mAb (bsm-52594R)  
~~~促銷,代碼KT202504~~~
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產(chǎn)品編號 bsm-52594R
英文名稱 AGR2 Recombinant Rabbit mAb
中文名稱 前梯度同源蛋白2重組兔單抗
別    名 HAG2; AG2 protein; hAG2; Secreted cement gland protein XAG2 homolog; AG 2; AG 2 protein; AG-2; AG2; AG2 protein; AGR 2; AGR2; AGR2_HUMAN; Anterior gradient 2 homolog(Xenopus laevis); anterior gradient 2 homolog; Anterior gradient protein 2 homolog; GOB 4; GOB4; HAG 2; hAG-2; hAG2; HAG2/R; HPC 8; HPC8; secreted cement gland homolog; Secreted cement gland protein XAG 2 homolog; Secreted cement gland protein XAG-2 homolog; Secreted cement gland protein XAG2 homolog; XAG 2; XAG2.  
研究領(lǐng)域 腫瘤  細胞生物  免疫學(xué)  轉(zhuǎn)錄調(diào)節(jié)因子  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 3F8
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1:20-100,ICC/IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 20 kDa
檢測分子量
細胞定位 細胞漿 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human AGR2 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 AGR2 is a member of the anterior gradient homolog family. It is the human ortholog of XAG-2, the secreted Xenopus laevis Anterior Gradient protein. In X. laevis, it is involved in cement gland differentiation and neural marker gene expression. AGR2 is a secretory protein encoded by two different AGR2 transcripts. It interacts with LYPD3 and ?dystroglycan (DAG-1). AGR2 is ubiquitously expressed with up-regulated expression in prostate cancer, breast cancer, lung cancer, renal carcinomas and endometrial carcinomas. AGR2 expression is positively correlated with that of the estrogen receptor (ER) and is negatively correlated with that of the epidermal growth factor receptor (EGFR). AGR2 may serve as a potential therapeutic marker for various cancers.

Function:
Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.

Subunit:
Interacts with LYPD3 and DAG1 (alphaDAG1). Interacts with MUC2; disulfide-linked.

Subcellular Location:
Secreted. Endoplasmic reticulum.

Tissue Specificity:
Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.

Similarity:
Belongs to the AGR family.

SWISS:
O95994

Gene ID:
10551

Database links:

Entrez Gene: 10551 Human

Entrez Gene: 23795 Mouse

Entrez Gene: 298961 Rat

Omim: 606358 Human

SwissProt: O95994 Human

SwissProt: O88312 Mouse

Unigene: 530009 Human

Unigene: 7244 Mouse

Unigene: 98889 Rat



產(chǎn)品圖片
25 ug total protein per lane of various lysates (see on figure) probed with AGR2 monoclonal antibody, unconjugated (bsm-52594R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AGR2 Monoclonal Antibody, Unconjugated(bsm-52594R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AGR2 Monoclonal Antibody, Unconjugated(bsm-52594R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AGR2 Monoclonal Antibody, Unconjugated(bsm-52594R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AGR2 Monoclonal Antibody, Unconjugated(bsm-52594R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AGR2 Monoclonal Antibody, Unconjugated(bsm-52594R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AGR2 Monoclonal Antibody, Unconjugated(bsm-52594R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AGR2 Monoclonal Antibody, Unconjugated(bsm-52594R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AGR2 Monoclonal Antibody, Unconjugated(bsm-52594R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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