| 產(chǎn)品編號 | bs-60295G-BF488 |
| 英文名稱 | AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L), BF488 conjugated |
| 中文名稱 | BF488標記山羊抗兔IgG(H+L), F(ab')2片段抗體 |
| 別 名 | BF488 AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L); F(ab)2 Fragment Goat Anti-Rabbit IgG H&L (BF488); F(ab)2 Fragment Goat Anti-Rabbit IgG H&L/BF488; F(ab')? Fragment Goat Anti-rabbit IgG H+L (BF488); BF488 AffiniPure F(ab')2 Fragment Go |
| 抗體來源 | |
| 克隆類型 | |
| 交叉反應(yīng) | Rabbit |
| 產(chǎn)品應(yīng)用 | IF=1:200-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 亞 型 | |
| 保存條件 | Store at -20 ℃. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
Target: Rabbit Host: Goat Antibody Format: F(ab')? Fragment Specificity: IgG (H+L) Minimal Cross Reactivity: Human, Mouse, Rat Serum Proteins Conjugate: Alexa Fluor? 488 (Amax: 493 Emax: 519nm) Product Category: F(ab')? Fragment Affinity-Purified Antibodies Dilution Range: 1:100-800 for most applications Purity: The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative: 0.05% Sodium Azide Information: Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, mouse and rat serum proteins, but it may cross-react with immunoglobulins from other species. F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G. |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
