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Rabbit Anti-Phospho-TFEB(Ser142)  antibody (bs-22337R)  
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產(chǎn)品編號 bs-22337R
英文名稱 Rabbit Anti-Phospho-TFEB(Ser142)  antibody
中文名稱 磷酸化T淋巴細胞轉錄調節(jié)因子TFEB抗體
別    名 TFEB(phospho-Ser142); TFEB(phospho-S142); TFEB(phospho Ser142); p-TFEB(Ser142); alpha TFEB; alphaTFEB; bHLHe35; T cell transcription factor EB; T-cell transcription factor EB; TCFEB; Transcription factor EB; TFEB_HUMAN.  
Specific References  (2)     |     bs-22337R has been referenced in 2 publications.
[IF=6.59] Dan Zhang. et al. Fagopyrum dibotrys extract alleviates hepatic steatosis and insulin resistance, and alters autophagy and gut microbiota diversity in mouse models of high-fat diet-induced non-alcoholic fatty liver disease. FRONT NUTR. 2022; 9: 993501  WB ;  Mouse.  
[IF=3.412] Hao Yu. et al. Activation of Transcription Factor EB Is Associated With Adipose Tissue Lipolysis in Dairy Cows With Subclinical Ketosis. Front Vet Sci. 2022; 9: 816064  WB ;  Cow(Calve).  
研究領域 細胞生物  免疫學  轉錄調節(jié)因子  t-淋巴細胞  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Dog,Horse)
產(chǎn)品應用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 52 kDa
檢測分子量
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human TFEB around the phosphorylation site of Ser142: PN(p-S)PM 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Transcription factors of the basic helix-loop-helix zipper (bHLH-ZIP) family contain a basic domain, used for DNA binding, and HLH and ZIP domains, both used for oligomerization. TFEB was isolated from a human B-cell cDNA library using a binding sequence from the adenovirus major late promoter. This transcription factor probably binds to the USF/MLTF site and probably recognizes E-box sequences in the heavy-chain immunoglobulin enhancer.

Function:
Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta.

Subunit:
Homodimer and heterodimer; with TFE3 or MITF.

Subcellular Location:
Cytoplasm. Nucleus. Note=Mainly present in the cytoplasm. Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus.

Post-translational modifications:
Sumoylated; does not affect dimerization with MITF.

Similarity:
Belongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain.

SWISS:
P19484

Gene ID:
7942

Database links:

Entrez Gene: 7942 Human

Entrez Gene: 510032 Cow

Entrez Gene: 609046 Dog

Entrez Gene: 21425 Mouse

Entrez Gene: 100157940 Pig

Entrez Gene: 316214 Rat

Omim: 600744 Human

SwissProt: P19484 Human

SwissProt: Q9R210 Mouse

Unigene: 485360 Human

Unigene: 715310 Human

Unigene: 2305 Mouse

Unigene: 22098 Rat



產(chǎn)品圖片
Sample: Pancreas (Mouse) Lysate at 40 ug Primary: Anti- Phospho-TFEB(Ser142) (bs-22337R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD
Sample: Lymph node(Mouse) Lysate at 40 ug Primary: Anti- Phospho-TFEB(Ser142) (bs-22337R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD
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