| 產(chǎn)品編號 | bs-23400R |
| 英文名稱 | TREM1 Rabbit pAb |
| 中文名稱 | 髓系細(xì)胞觸發(fā)受體1抗體 |
| 別 名 | Triggering receptor expressed on myeloid cells 1; TREM 1; TREM-1; Triggering receptor expressed on monocytes 1; Triggering receptor TREM 1; Triggering receptor TREM1; Triggering-receptor TREM 1; Triggering-receptor TREM-1; Triggering-receptor TREM1; TREM1 |
| 研究領(lǐng)域 | 免疫學(xué) 細(xì)胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 24 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse TREM1: 1-100/230 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]. Function: Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock (By similarity). Subunit: Interacts with TYROBP/DAP12. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein (Potential). Isoform 2: Secreted (Potential). Tissue Specificity: Highly expressed in adult liver, lung and spleen than in corresponding fetal tissue. Also expressed in the lymph node, placenta, spinal cord and heart tissues. Expression is more elevated in peripheral blood leukocytes than in the bone marrow and in normal cells than malignant cells. Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Strongly expressed in acute inflammatory lesions caused by bacteria and fungi. Isoform 2 was detected in the lung, liver and mature monocytes. Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q9JKE2 Gene ID: 58217 Database links: Entrez Gene: 54210 Human Entrez Gene: 58217 Mouse Omim: 605085 Human SwissProt: Q9NP99 Human SwissProt: Q9JKE2 Mouse Unigene: 283022 Human Unigene: 248352 Mouse |
| 產(chǎn)品圖片 |
Sample:
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti-TREM1 (bs-23400R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 29 kD
Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TREM1) Polyclonal Antibody, Unconjugated (bs-23400R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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