| 產(chǎn)品編號 | bs-33206R |
| 英文名稱 | S1-Tag Rabbit pAb |
| 中文名稱 | S1標簽抗體 |
| 別 名 | S1 Tag; S1Tag; NANNPDWDF; |
| 產(chǎn)品類型 | 標簽抗體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Recombinant protein |
| 產(chǎn)品應(yīng)用 | WB=1:1000-5000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide S1-Tag |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Epitope tagging is a recombinant DNA method by which a protein encoded by a cloned gene is made immunoreactive to a known antibody. Major areas ofapplication include monitoring protein expression, localizing proteins at thecellular and subcellular levels, and protein purification, as well as the analysis of protein topology, dynamics and interactions. Due to their small size, theyare unlikely to affect the tagged protein's biochemical properties.S1 tag is an epitope tag composed of a nine-residue peptide, NANNPDWDF, derived from the hepatitis B virus preS1 region. Epitope tags consisting ofshort sequences recognized by well-characterizated antibodies have beenwidely used in the study of protein expression in various systems. |
| 產(chǎn)品圖片 |
Sample:
Lane 1: S1-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug
Lane 2: S1-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug
Primary: Anti-S1-Tag (bs-33206R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
