| 產(chǎn)品編號 | bsm-33111M |
| 英文名稱 | FUT4 Mouse mAb |
| 中文名稱 | α-(1,3)-巖藻糖基轉(zhuǎn)移酶 4單克隆抗體 |
| 別 名 | FUT4_HUMAN; alpha-(1, 3)-fucosyltransferase 4; 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; EC:2.4.1.152; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV(Fuc-TIV; FucT-IV); Galactoside 3-L-fucosyltransfera |
| 研究領域 | 腫瘤 細胞生物 免疫學 細胞膜受體 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 8B9 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 58 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FUT4 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq]. Function: May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. Subcellular Location: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. Tissue Specificity: Highest expression in stomach and colon. It is also expressed in the lung, testis, uterus, small intestine and to a lesser extent in spleen, and ovary. Present in trace amounts in brain, thymus, heart, smooth muscle, kidney and bone marrow. Not found in liver, salivary gland and pancreas. Similarity: Belongs to the glycosyltransferase 10 family. SWISS: P22083 Gene ID: 2526 Database links: Entrez Gene: 10690 Human Entrez Gene: 2526 Human Entrez Gene: 2527 Human Entrez Gene: 2528 Human Entrez Gene: 2529 Human Omim: 104230 Human SwissProt: P22083 Human SwissProt: P51993 Human SwissProt: Q11128 Human SwissProt: Q11130 Human SwissProt: Q9Y231 Human Unigene: 390420 Human Unigene: 457 Human Unigene: 49117 Human Unigene: 572064 Human Unigene: 623098 Human Unigene: 631843 Human Unigene: 631846 Human Unigene: 705615 Human FUT4，也稱為 ELFT 和 FCT3A，屬于糖基轉(zhuǎn)移酶 10 家族。FUT4 可催化參與 Lewis X/SSEA-1 和 VIM-2 抗原表達的 alpha-1,3 糖苷鍵。FUT4 是一種抗原表位，定義為 Lewis X 碳水化合物結(jié)構(gòu)，在鼠胚胎癌細胞 (EC)、鼠 ES 和 iPS 細胞以及鼠和人類生殖細胞上表達。它被廣泛用作小鼠未分化 ES 和 iPS 細胞的陽性表面標志物和人未分化 ES 和 iPS 細胞的陰性表面標志物。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human esophageal); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FUT4) Monoclonal Antibody, Unconjugated (ascites of bsm-33111M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FUT4) Monoclonal Antibody, Unconjugated (ascites of bsm-33111M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
