| 產(chǎn)品編號 | bs-20325R |
| 英文名稱 | EAAT2 Rabbit pAb |
| 中文名稱 | 膠質(zhì)細胞谷氨酸運載蛋白2抗體 |
| 別 名 | EAAT2; Excitatory amino acid transporter 2; Excitotoxic amino acid transporter 2; Glial high affinity glutamate transporter; GLT 1; GLT1; Glutamate aspartate transporter II; SLC1A2; Sodium dependent glutamate aspartate transporter 2; Solute carrier family |
 |
Specific References (1) | bs-20325R has been referenced in 1 publications.
[IF=3.606] Yingjiao Liuet al. Exogenous Adenosine Antagonizes Excitatory Amino Acid Toxicity in Primary Astrocytes. Cell Mol Neurobiol
. 2020 Jul 6. WB, IF ; rat.
|
| 研究領(lǐng)域 | 免疫學 神經(jīng)生物學 通道蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Rabbit,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 62 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EAAT2: 351-450/574 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Sep 2010] Function: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Subunit : Homotrimer. Interacts with AJUBA. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Brain Post-translational modifications: Glycosylated. Palmitoylation at Cys-38 is not required for correct subcellular localization, but is important for glutamate uptake activity. Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily. SWISS: P43004 Gene ID: 6506 Database links: Entrez Gene: 6506 Human Entrez Gene: 20511 Mouse Omim: 600300 Human SwissProt: P43004 Human SwissProt: P43006 Mouse Unigene: 502338 Human Unigene: 267547 Mouse Unigene: 371582 Mouse Unigene: 10240 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human U251 cell lysates
Lane 2: Human SH-SY5Y cell lysates
Lane 3: Human HepG2 cell lysates
Primary: Anti-EAAT2 (bs-20325R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 66 kDa
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EAAT2) Polyclonal Antibody, Unconjugated (bs-20325R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
