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Rabbit Anti-SLC25A11  antibody (bs-8703R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號(hào) bs-8703R
英文名稱 Rabbit Anti-SLC25A11  antibody
中文名稱 溶質(zhì)載體家族25成員11抗體
別    名 M2OM_HUMAN; Mitochondrial 2 oxoglutarate/malate carrier protein; Mitochondrial 2-oxoglutarate/malate carrier protein; OGC; OGCP; SLC20A4; Solute carrier family 20 (oxoglutarate carrier) member 4; Solute carrier family 20 member 4; Solute carrier family 25 (mitochondrial carrier oxoglutarate carrier) member 11; Solute carrier family 25 member 11.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  信號(hào)轉(zhuǎn)導(dǎo)  轉(zhuǎn)運(yùn)蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse (predicted: Rat,Rabbit,Sheep,Cow,GuineaPig)
產(chǎn)品應(yīng)用 WB=1:500-2000,Flow-Cyt=1ug/Test,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 34kDa
細(xì)胞定位 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SLC25A15: 101-200/314 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The oxoglutarate/malate carrier transports 2-oxoglutarate across the inner membranes of mitochondria in an electroneutral exchange for malate or other dicarboxylic acids (summary by Iacobazzi et al., 1992 [PubMed 1457818]).[supplied by OMIM, Jan 2011]

Function:
Catalyzes the transport of 2-oxoglutarate across the inner mitochondrial membrane in an electroneutral exchange for malate or other dicarboxylic acids, and plays an important role in several metabolic processes, including the malate-aspartate shuttle, the oxoglutarate/isocitrate shuttle, in gluconeogenesis from lactate, and in nitrogen metabolism.

Subcellular Location:
Mitochondrion inner membrane.

Similarity:
Belongs to the mitochondrial carrier family.
Contains 3 Solcar repeats.

SWISS:
Q02978

Gene ID:
8402

Database links:

Entrez Gene: 8402 Human

Entrez Gene: 67863 Mouse

Entrez Gene: 64201 Rat

Omim: 604165 Human

SwissProt: Q02978 Human

SwissProt: Q9CR62 Mouse

SwissProt: P97700 Rat

Unigene: 184877 Human

Unigene: 296082 Mouse

Unigene: 466994 Mouse

Unigene: 853 Rat



產(chǎn)品圖片
Sample: Lane 1: Human U251 cell lysates Lane 2: Human A549 cell lysates Primary: Anti-SLC25A11 (bs-8703R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 32 kDa
Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-SLC25A15 (bs-8703R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 34 kD
Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-SLC25A15 antibody (bs-8703R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST methanol for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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