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Rabbit Anti-HIP55  antibody (bs-20165R)  
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產(chǎn)品編號 bs-20165R
英文名稱 Rabbit Anti-HIP55  antibody
中文名稱 宮頸黏液蛋白相關(guān)蛋白抗體
別    名 ABP1; Actin-binding protein 1; Cervical mucin associated protein; Cervical mucin-associated protein; Cervical SH3P7; CMAP; Dbnl; DBNL_HUMAN; Drebrin F; drebrin like; Drebrin like protein; Drebrin-F; Drebrin-like protein; HIP 55; HIP-55; HPK1 interacting protein of 55 kDa; HPK1-interacting protein of 55 kDa; SH3 domain containing protein 7; SH3 domain-containing protein 7; SH3P7; Src Homology 3 Domain Containing Protein; src homology 3 domain containing protein HIP.  
研究領(lǐng)域 信號轉(zhuǎn)導(dǎo)  轉(zhuǎn)運(yùn)蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Mouse (predicted: Human)
產(chǎn)品應(yīng)用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 55 kDa
檢測分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human HIP55: 201-300/430 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Drebrins (developmentally regulated brain proteins) are cytoplasmic proteins that bind F-actin in the brain and are involved in cell migration, extension of neuronal processes and plasticity of dendrites. HIP-55 (HPK1-interacting protein of 55 kDa), also known as ABP1, SH3P7 or DBNL (drebrin-like), is a 430 amino acid cytoplasmic protein that belongs to the ABP1 family. HIP-55 binds to F-actin but is not involved in actin polymerization, capping or bundling. In addition to containing an ADF-H domain, HIP-55 also consists of a SH3 domain, which mediates interaction with SHANK2, SHANK3 and PRAM-1. HIP-55 acts as an actin-binding adapter protein and as a common effector of antigen receptor-signaling pathways in leukocytes. As a key component of the immunological synapse, HIP-55 regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. HIP-55 is degraded by caspases during apoptosis.

Function:
Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin.

Subunit:
Interacts with SHANK2 and SHANK3. Interacts with FGD1, dynamin, MAP4K1 and PRAM1. Interacts with ANKRD54.

Subcellular Location:
Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Note=Cortical cytoskeleton. Associates with lamellipodial actin.

Post-translational modifications:
Degraded by caspases during apoptosis.

Similarity:
Belongs to the ABP1 family.
Contains 1 ADF-H domain.
Contains 1 SH3 domain.

SWISS:
Q9UJU6

Gene ID:
28988

Database links:

Entrez Gene: 28988 Human

Entrez Gene: 13169 Mouse

Entrez Gene: 83527 Rat

Omim: 610106 Human

SwissProt: Q9UJU6 Human

SwissProt: Q62418 Mouse

SwissProt: Q9JHL4 Rat

Unigene: 436500 Human

Unigene: 256925 Mouse

Unigene: 4115 Rat



產(chǎn)品圖片
Protein: lymph node(mouse) lyates at 40ug; spleen(mouse) lyates at 40ug; Primary: Rabbit Anti-HIP55 (bs-20165R) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:55 kD Observed band size:55 kD
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