| 產(chǎn)品編號(hào) | bs-20131R |
| 英文名稱 | Rabbit Anti-DLC1 antibody |
| 中文名稱 | 肝癌缺失基因1抗體 |
| 別 名 | ARHGAP 7; ARHGAP7; Deleted in liver cancer 1; Deleted in liver cancer 1 protein; DLC 1; Dlc-1; DLC1; DLC1 Rho GTPase activating protein; FLJ21120; HP; HP protein; KIAA1723; p122 RhoGAP; RHG07_HUMAN; Rho GTPase activating protein 7; Rho GTPase-activating protein 7; Rho type GTPase activating protein 7; Rho-type GTPase-activating protein 7; StAR related lipid transfer (START) domain containing 12; StAR related lipid transfer protein 12; StAR-related lipid transfer protein 12; STARD 12; StARD12; START domain containing protein 12; START domain-containing protein 12. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 線粒體 表觀遺傳學(xué) G蛋白信號(hào) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 171kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DLC1: 601-700/1528 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a GTPase-activating protein (GAP) that is a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins. GAP family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. This gene functions as a tumor suppressor gene in a number of common cancers, including prostate, lung, colorectal, and breast cancers. Multiple transcript variants due to alternative promoters and alternative splicing have been found for this gene.[provided by RefSeq, Apr 2010]. Function: Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality. Subunit: Interacts with EF1A1, facilitates EF1A1 distribution to the membrane periphery and ruffles upon growth factor stimulation and suppresses cell migration. Subcellular Location: Cytoplasm. Cell junction, focal adhesion. Membrane; Peripheral membrane protein. Note=Colocalizes with EF1A1 at actin-rich regions in the cell periphery. Tissue Specificity: Highest level of expression in the spleen, with rather lower levels in prostate, testis, ovary, small intestine and colon, but none in the thymus. Post-translational modifications: Phosphorylation at Ser-88 appears to control the dimer-monomer transition. According to PubMed:15193260, it is phosphorylated at Ser-88 by PAK1, however, according to PubMed:18650427, the DYNLL1 dimer is not accessible for PAK1 and the phosphorylation could not be demonstrated in vitro. Similarity: Contains 1 Rho-GAP domain. Contains 1 SAM (sterile alpha motif) domain. Contains 1 START domain. SWISS: Q96QB1 Gene ID: 10395 Database links: Entrez Gene: 10395 Human Entrez Gene: 50768 Mouse Omim: 604258 Human SwissProt: Q96QB1 Human SwissProt: Q9R0Z9 Mouse Unigene: 134296 Human Unigene: 210875 Mouse Unigene: 224563 Rat |
| 產(chǎn)品圖片 |
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-DLC1 (bs-20131R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 171 kD
Observed band size: 125 kD
Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DLC1 Polyclonal Antibody, Unconjugated(bs-20131R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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