| 產(chǎn)品編號 | bs-19159R |
| 英文名稱 | Rabbit Anti-ZFYVE26 antibody |
| 中文名稱 | 鋅指蛋白結(jié)構(gòu)域ZFYVE26抗體 |
| 別 名 | DKFZp686F19106; DKFZp781H1112; FYVE domain-containing centrosomal protein; FYVE-CENT; KIAA0321; Spastic paraplegia 15(complicated autosomal recessive); Spastizin; SPG15; ZFY26_HUMAN; ZFYVE 26; ZFYVE26; Zinc finger FYVE domain containing 26; Zinc finger FYVE domain containing protein 26; Zinc finger FYVE domain-containing protein 26. |
| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 神經(jīng)生物學(xué) 鋅指蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 285 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZFYVE26: 2151-2250/2539 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a protein which contains a FYVE zinc finger binding domain. The presence of this domain is thought to target these proteins to membrane lipids through interaction with phospholipids in the membrane. Mutations in this gene are associated with autosomal recessive spastic paraplegia-15. [provided by RefSeq, Oct 2008] Function: Phosphatidylinositol 3-phosphate-binding protein required for the abcission step in cytokinesis: recruited to the midbody during cytokinesis and acts as a regulator of abcission. May also be required for efficient homologous recombination DNA double-strand break repair. Subcellular Location: Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Midbody. Localizes to the centrosome during all stages of the cell cycle. Recruited to the midbody during cytokinesis by KIF13A. Tissue Specificity: Strongest expression in the adrenal gland, bone marrow, adult brain, fetal brain, lung, placenta, prostate, skeletal muscle, testis, thymus, and retina. Intermediate levels are detected in other structures, including the spinal cord. DISEASE: Spastic paraplegia 15, autosomal recessive Similarity: Contains 1 FYVE-type zinc finger. SWISS: Q68DK2 Gene ID: 23503 Database links: Entrez Gene: 23503 Human Entrez Gene: 211978 Mouse Omim: 612012 Human SwissProt: Q68DK2 Human SwissProt: Q5DU37 Mouse Unigene: 98041 Human Unigene: 297919 Mouse Unigene: 16048 Rat |
| 產(chǎn)品圖片 |
Sample:
Brain (Mouse) Lysate at 40 ug
U251 (Human) CellLysate at 30 ug
U87MG (Human) CellLysate at 30 ug
Primary: Anti-ZFYVE26 (bs-19159R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 285 kD
Observed band size: 285 kD
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti-ZFYVE26 (bs-19159R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 285 kD
Observed band size: 285 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZFYVE26) Polyclonal Antibody, Unconjugated (bs-19159R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
