| 產(chǎn)品編號(hào) | bs-18704R |
| 英文名稱 | MBD4 Rabbit pAb |
| 中文名稱 | 甲基化CpG結(jié)合蛋白MBD4抗體 |
| 別 名 | 3 N(4) ethenocytosine glycosylase; G/5 fluorouracil mismatch glycosylase with biphasic kinetics; G/T mismatch glycosylase; G/U mismatch glycosylase; MBD 4; MBD4; MBD4_HUMAN; MED 1; MED1; Methyl CpG binding domain protein 4; Methyl CpG binding endonuclease |
| 研究領(lǐng)域 | 細(xì)胞生物 轉(zhuǎn)錄調(diào)節(jié)因子 結(jié)合蛋白 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Pig,Sheep,Cow) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 66 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MBD4: 361-460/580 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013] Function: Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein. Subcellular Location: Nucleus. Similarity: Contains 1 MBD (methyl-CpG-binding) domain. SWISS: O95243 Gene ID: 8930 Database links: Entrez Gene: 8930 Human Entrez Gene: 17193 Mouse Omim: 603574 Human SwissProt: O95243 Human SwissProt: Q9Z2D7 Mouse Unigene: 35947 Human Unigene: 259308 Mouse |
| 產(chǎn)品圖片 |
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-MBD4 (bs-18704R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66kD
Observed band size: 66 kD
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