| 產(chǎn)品編號 | bs-17652R |
| 英文名稱 | SPEM1 Rabbit pAb |
| 中文名稱 | 精子細胞成熟蛋白1抗體 |
| 別 名 | C17orf83; Spem1; SPEM1_HUMAN; Spermatid maturation 1; Spermatid maturation protein 1. |
| 研究領(lǐng)域 | 細胞生物 發(fā)育生物學(xué) 跨膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human,Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 35 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SPEM1: 201-300/309 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
SPEM1 is a 309 amino acid transmembrane and cytoplasmic protein that is required for proper cytoplasm removal during spermatogenesis. SPEM1 interacts with both Ran BP-17 and PLIC-1. Since PLIC-1 functions through binding and directing poly-ubiquitinated proteins to the proteasome for degradation, interactions between PLIC-1 and SPEM1 suggest a role in the regulation of protein ubiquitination during spermiogenesis. The SPEM1 gene maps to human chromosome 17p13.1. Comprising over 2.5% of the human genome, chromosome 17 consists of about 81 million bases, encodes over 1,200 genes and has the highest gene density in the genome. Chromosome 17 is also enriched in segmental duplications, ranking third in density among the autosomes. Function: Required for proper cytoplasm removal during spermatogenesis. Subcellular Location: Membrane. Cytoplasm. SWISS: Q8N4L4 Gene ID: 374768 Database links: Entrez Gene: 374768 Human SwissProt: Q8N4L4 Human Unigene: 710599 Human |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
