| 產品編號 | bs-17636R |
| 英文名稱 | Rabbit Anti-SPATA15/SPATC1 antibody |
| 中文名稱 | 精子發(fā)生相關蛋白15抗體 |
| 別 名 | 1700084J23Rik; MGC144830; MGC61633; SPATA15; Spatc1; SPERI_HUMAN; Speriolin; Spermatogenesis and centriole associated 1; Spermatogenesis and centriole-associated protein 1; Spermatogenesis-associated protein 15; Spermatogenic cell specific Cdc20 binding protein; Spermatogenic cell-specific Cdc20-binding protein. |
| 研究領域 | 細胞生物 發(fā)育生物學 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Sheep,Cow,Horse) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 62kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SPATA15/SPATC1: 1-100/591 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
SPATA15 is a 591 amino acid cytoplasmic protein expressed in testis that is part of a complex that includes p55 CDC, Cdc27. The gene encoding Speriolin is located on human chromosome 8, which consists of nearly 146 million base pairs, houses more than 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that map to chromosome 8. Subunit: Found in a complex with CDC20, CDC27 and TUBG1. Interacts with CDC20 (By similarity). Subcellular Location: Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Colocalizes with the centrosomal pericentrin protein PCNT1. Tissue Specificity: Detected only in testis. SWISS: Q76KD6 Gene ID: 375686 Database links: Entrez Gene: 375686 Human Entrez Gene: 74281 Mouse Omim: 610874 Human SwissProt: Q76KD6 Human SwissProt: Q148B6 Mouse Unigene: 97726 Human |
| 產品圖片 |
Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti-SPATA15/SPATC1(bs-17636R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62kD
Observed band size: 62kD
Sample: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-SPATA15/SPATC1(bs-17636R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62kD
Observed band size: 62kD
Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti-SPATA15/SPATC1(bs-17636R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62kD
Observed band size: 62kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
