| 產(chǎn)品編號 | bs-17326R |
| 英文名稱 | LULL1/TOR1AIP2 Rabbit pAb |
| 中文名稱 | LULL1蛋白抗體 |
| 別 名 | TOR1AIP2; IFRG15; Interferon responsive gene 15; LULL1; Lumenal domain like LAP1; NET9; RP11-12M5.5; TOR1AIP2; Torsin 1A interacting protein 2; Torsin A interacting protein 2. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 51 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LULL1: 221-320/470 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
LULL1 is a 470 amino acid endoplasmic reticulum single-pass membrane protein belonging to the TOR1AIP family. LULL1 interacts with torsinA, an essential AAA+ ATPase found in the endoplasmic reticulum (ER) and nuclear envelope (NE) of higher eukaryotes. LULL1 regulates the distribution and activity of torsinA within the ER and NE lumen and reveals functional defects in mutant torsinA, which is responsible for DYT1 dystonia, a neurodevelopmental disease caused by an in-frame deletion (Deltagag) in the gene encoding torsinA. The gene encoding LULL1 maps to human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome. Function: Regulates the distribution of TOR1A between the endoplasmic reticulum and the nuclear envelope. Subunit: Interacts with TOR1A and TOR1B (ATP-bound). Subcellular Location: Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane. SWISS: Q8NFQ8 Gene ID: 163590 Database links: Entrez Gene: 163590 Human Omim: 614513 Human SwissProt: Q8NFQ8 Human |
| 產(chǎn)品圖片 |
Sample: Small intestine (Mouse) Lysate at 40 ug
Primary: Anti-LULL1/TOR1AIP2(bs-17326R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51kD
Observed band size: 56kD
Sample:
Placenta (Mouse) Lysate at 40 ug
Large intestine (Mouse) Lysate at 40 ug
Primary: Anti-LULL1/TOR1AIP2(bs-17326R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51kD
Observed band size: 56kD
Sample:
Lane 1: K562 (Human) Cell Lysate at 30 ug
Lane 2: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 3: Hela (Human) Cell Lysate at 30 ug
Lane 4: HUVEC (Human) Cell Lysate at 30 ug
Lane 5: U2os (Human) Cell Lysate at 30 ug
Lane 6: HepG2 (Human) Cell Lysate at 30 ug
Lane 7: Liver (Mouse) Lysate at 40 ug
Lane 8: Liver (Rat) Lysate at 40 ug
Lane 9: Kidney (Mouse) Lysate at 40 ug
Lane 10: Cerebrum (Mouse) Lysate at 40 ug
Lane 11: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-LULL1/TOR1AIP2 (bs-17326R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51kD
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LULL1) Polyclonal Antibody, Unconjugated (bs-17326R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LULL1) Polyclonal Antibody, Unconjugated (bs-17326R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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