| 產(chǎn)品編號(hào) | bs-16868R |
| 英文名稱 | phospho-KSR1 (Ser404) Rabbit pAb |
| 中文名稱 | 磷酸化Ras信號(hào)轉(zhuǎn)導(dǎo)KSR1蛋白抗體 |
| 別 名 | KSR1(phospho S404); p-KSR1(phospho S404); BKSR 1; BKSR1; dKsr; EK 31; EK31; hb; Kinase suppressor of Ras 1; Kinase suppressor of ras; KSR 1; KSR; KSR1; KSR1_HUMAN; Positive Ras signaling mediator family member(86.4 kD); Positive Ras signaling mediator fam |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Pig,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 102 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human KSR1 around the phosphorylation site of Ser404: TE(p-S)VP |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
KSR1 is a molecular scaffold and positive regulator of the Raf/MEK/ERK phosphorylation cascade. It was initially considered to be a protein kinase acting in the Ras pathway. KSR1 is required for maximal ERK activation induced by growth factors and by some cytotoxic agents. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane associated. Function: Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity. Subcellular Location: Cytoplasm. Membrane. In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery. Post-translational modifications: Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated. Similarity: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain. SWISS: Q8IVT5 Gene ID: 8844 Database links: Entrez Gene: 8844 Human Omim: 601132 Human SwissProt: Q8IVT5 Human Unigene: 133534 Human |
| 產(chǎn)品圖片 |
Hela (H) cells were treated with or without Calyculin A (50nM 30) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-KSR1 (Ser404) polyclonal antibody, unconjugated (bs-16868R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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