| 產(chǎn)品編號 | bs-14668R |
| 英文名稱 | Rabbit Anti-EXOSC5 antibody |
| 中文名稱 | EXOSC5蛋白抗體 |
| 別 名 | Chronic myelogenous leukemia tumor antigen 28; CML28; EXOS5_HUMAN; EXOSC 5; Exosc5; Exosome complex component rrp46; exosome complex exonuclease RRP46; Exosome component 5; exosome component Rrp46; hRrp46p; p12B; Ribosomal RNA processing protein 46; Ribosomal RNA processing protein 46, S. cerevisiae, homolog of; Ribosomal RNA-processing protein 46; RRP41B; RRP46; Rrp46p. |
| 研究領(lǐng)域 | 細(xì)胞生物 轉(zhuǎn)運蛋白 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 25kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EXOSC5: 141-235/235 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The exosome is a multisubunit complex composed of several highly conserved subunits, some of which are 3’ to 5’ exoribonucleases. The complex is involved in a variety of cellular processes and is responsible for degrading unstable mRNAs that contain AU-rich (ARE) elements in their untranslated 3’ region. EXOSC5 (Exosome component 5), also known as Exosome complex exonuclease RRP46 and CML28 (Chronic myelogenous leukemia tumor antigen 28), is one of at least eleven components of the exosome complex and is required for processing of 7S pre-RNA to mature 5.8S rRNA. Located in the nucleus, EXOSC5 interacts with both EXOSC1 and EXOSC7. EXOSC5 is exclusively expressed in normal testis, though is found to be highly expressed in many hematopoietic and epithelial tumor cell lines, suggesting that it may be an appropriate target for antigen-specific immunotherapy. Function: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. Subcellular Location: Nucleus; nucleolus. Cytoplasm. Nucleus. Tissue Specificity: Highly expressed in a variety of hematopoietic and epithelial tumor cell lines, but not in normal hematopoietic tissues or other normal tissue, with the exception of testis. Similarity: Belongs to the RNase PH family. SWISS: Q9NQT4 Gene ID: 56915 Database links: Entrez Gene: 56915 Human Entrez Gene: 27998 Mouse Omim: 606492 Human SwissProt: Q9NQT4 Human SwissProt: Q9CRA8 Mouse Unigene: 283741 Human Unigene: 207484 Mouse Unigene: 27853 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EXOSC5) Polyclonal Antibody, Unconjugated (bs-14668R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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